Tissue-engineered oral mucosa to study radiotherapy-induced oral mucositis

Colley, Helen E., Eves, Paula, Pinnock, Abigail, Thornhill, Martin H. and Murdoch, Craig (2013) Tissue-engineered oral mucosa to study radiotherapy-induced oral mucositis. International Journal of Radiation Biology, 89 (11). pp. 907-914. ISSN 0955-3002

Others
09553002.2013.809171_journalCode=irab20&
[img]
[Download]
[img] XML
09553002.2013.809171_journalCode=irab20&

98kB
Item Type:Article
Item Status:Live Archive

Abstract

Purpose: Oral mucositis is a severe and often dose-limiting side-effect of cancer therapy that occurs in patients receiving radiotherapy for head and neck cancers. Although radiation-induced effects on keratinocytes have been studied, little is known about its effect on fibroblasts or endothelial cells or, more importantly, when all these cells are combined in an engineered oral mucosal model. Materials and methods: Monolayer cultures of normal oral keratinocytes, normal oral fibroblasts, human dermal microvascular endothelial cells or tissue-engineered oral mucosa (TEOM) were exposed to 20 Gy irradiation. Cell damage and cytokine release was measured for 72 h for monolayer cultures and for up to 21 d for TEOM. Results: Compared to non-irradiated cells, the viability of all monolayer and co-cultures was significantly reduced 72 h post-irradiation while levels of secreted interleukin IL-6 and CXCL8 were increased. The viability of irradiated TEOM models was significantly reduced compared to controls at all time-points. Histologically, irradiated TEOM displayed thinner epithelium, increased apoptosis and more extensive damage than non-irradiated models. IL-6, CXCL8 and granulocyte macrophage colony-stimulating factor release was reduced whereas IL-1α levels were increased in irradiated TEOM models compared to controls. Conclusions: TEOM models comprising of mixed cell populations may prove useful in examining the pathobiology of radiation-induced mucositis. © 2013 Informa UK, Ltd.

Keywords:Mucositis, cytokine, head and neck cancer, Oral Cancer, Radiation
Subjects:A Medicine and Dentistry > A100 Pre-clinical Medicine
C Biological Sciences > C131 Applied Cell Biology
A Medicine and Dentistry > A200 Pre-clinical Dentistry
Divisions:Professional services
Related URLs:
ID Code:26982
Deposited On:24 Apr 2017 10:18

Repository Staff Only: item control page