Nolan, Aoife M., Collins, Louise M., Wyatt, Sean L. , Gutierrez, Humberto and O'Keeffe, Gerard W. (2014) The neurite growth inhibitory effects of soluble TNFα on developing sympathetic neurons are dependent on developmental age. Differentiation, 88 (4-5). pp. 124-130. ISSN 0301-4681
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Item Type: | Article |
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Item Status: | Live Archive |
Abstract
During development, the growth of neural processes is regulated by an array of cellular and molecular mechanisms which influence growth rate, direction and branching. Recently, many members of the TNF superfamily have been shown to be key regulators of neurite growth during development. The founder member of this family, TNFα can both promote and inhibit neurite growth depending on the cellular context. Specifically, transmembrane TNFα promotes neurite growth, while soluble TNFα inhibits it. While the growth promoting effects of TNFα are restricted to a defined developmental window of early postnatal development, whether the growth inhibitory effects of soluble TNFα occur throughout development is unknown. In this study we used the extensively studied, well characterised neurons of the superior cervical ganglion to show that the growth inhibitory effects of soluble TNFα are restricted to a specific period of late embryonic and early postnatal development. Furthermore, we show that this growth inhibitory effect of soluble TNFα requires NF-κB signalling at all developmental stages at which soluble TNFα inhibits neurite growth. These findings raise the possibility that increases in the amount of soluble TNFα in vivo, for example as a result of maternal inflammation, could negatively affect neurite growth in developing neurons at specific stages of development.
Keywords: | immunoglobulin enhancer binding protein, messenger RNA, tumor necrosis factor alpha, tumor necrosis factor receptor 1, tumor necrosis factor receptor 2, animal cell, Article, cancer inhibition, cell survival, cellular distribution, controlled study, developmental stage, embryo, embryo development, nerve cell culture, nerve cell differentiation, nerve fiber growth, nervous system development, nonhuman, postnatal development, protein determination, protein expression, protein localization, protein protein interaction, rat, real time polymerase chain reaction, signal transduction, superior cervical ganglion, sympathetic nerve cell, transcription initiation, transcription regulation, JCNotOpen |
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Subjects: | C Biological Sciences > C130 Cell Biology C Biological Sciences > C141 Developmental Biology |
Divisions: | College of Science > School of Life Sciences |
Related URLs: | |
ID Code: | 16879 |
Deposited On: | 04 Mar 2015 16:40 |
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