Comparison of different Solid Phase Micro Extraction (SPME) fibres for detection of selected 8-carbon compounds associated with fresh whole mushroom (Agaricus bisporus)

Daramola, Bukola, Fothergill, James, Taylor, Tony , Valverde, Juan and Warby, Jenna (2014) Comparison of different Solid Phase Micro Extraction (SPME) fibres for detection of selected 8-carbon compounds associated with fresh whole mushroom (Agaricus bisporus). In: Food Analysis Congress, 28th - 29th October 2014, Barcelona, Spain.

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Item Type:Conference or Workshop contribution (Poster)
Item Status:Live Archive

Abstract

In this study, three commercially available SPME fibres coated with different stationary phases were compared as regards to the detection of selected compounds associated with the headspace of fresh Agaricus bisporus. A 50/30 µm Divinylbenzene/Carboxen/Polydimethylsiloxane (DCP), 85 µm polyacrylate-coated fibre (PA) and 60 µm Polydimethylsiloxane/divinylbenzene fibre (PDMS/DVB), were exposed individually to 2 to 3 day old mushroom for 60 minutes and then desorbed with a GC-MS system for chromatographic separation for 31 minutes. Results obtained showed similar chromatogram pattern for DCP and PDMS fibre; however pattern for PA fibre differed significantly. Maximum recoveries for DCP and PDMS/DVB were between 6 and 14 minutes while recovery of organic compounds for PA occurred between 4 and 25 minutes. Among the 8-carbon compounds detected in whole fresh mushrooms; 1-octene, octane, 3-octanone and 4-methyloctane were detected with the use of DCP and PDMS/DVB. Although, the use of PA fibre also allowed for the detection of 3-octanone, PA fibre exhibited lowest extraction efficiency for this compound. As compared to DCP and PDMS/DVB which extract non polar compounds better, the PA fibre allowed for the detection of more polar compound such as octanoic acid, and 8-carbon saturated fatty acid.

Keywords:SPME fibre, Head space analysis, GC-MS, 8-Carbon compounds, Mushrooms, PA fibre, DCP fibre
Subjects:F Physical Sciences > F100 Chemistry
F Physical Sciences > F180 Analytical Chemistry
Divisions:College of Science > National Centre for Food Manufacturing
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ID Code:16388
Deposited On:08 Jan 2015 12:57

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