IL-1beta-dependent activation of NF-kappaB mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase

Catley, Matthew C., Chivers, Joanna E., Cambridge, Lisa M. , Holden, Neil, Slater, Donna M., Staples, Karl J., Bergmann, Martin W., Loser, Peter, Barnes, Peter J. and Newton, Robert (2003) IL-1beta-dependent activation of NF-kappaB mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase. FEBS Letters, 547 (1-3). pp. 75-79. ISSN 0014-5793

Full content URL: http://dx.doi.org/10.1016/S0014-5793(03)00672-0

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Item Type:Article
Item Status:Live Archive

Abstract

Prostaglandin (PG) E2 release is induced in pulmonary A549 cells by the NF-kappaB-activating stimuli interleukin-1beta (IL-1beta) and phorbol 12-myristate 13-acetate (PMA). Adenoviral over-expression of IkappaBalphaDeltaN, a dominant NF-kappaB inhibitor, prevents NF-kappaB-dependent transcription and was used to qualify the role of NF-kappaB in the release of PGE2. IkappaBalphaDeltaN repressed IL-1beta-induced, but not PMA-induced, cycloxygenase-2 (COX-2) and microsomal prostaglandin E synthase (mPGES) expression. These data conclusively demonstrate a substantial role for NF-kappaB in the co-ordinate induction of COX-2, mPGES and in the corresponding release of PGE2 by IL-1beta. However, other pathways are primarily responsible for PGE2 release induced by PMA.

Keywords:Prostaglandins, Prostaglandin E synthetase, Nuclear factor ?B, Prostaglandin GH synthase, Cyclooxygenase-2
Divisions:College of Science > School of Life Sciences
ID Code:15133
Deposited On:09 Jun 2015 08:30

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