Stratton, Rebecca C., Squires, Paul E. and Green, Anne K. (2008) ANP stimulates hepatocyte Ca2+ efflux via plasma membrane recruitment of PKGIα. Biochemical and Biophysical Research Communications, 36 (4). pp. 965-970. ISSN 0006-291X
Full content URL: http://dx.doi.org/10.1016/j.bbrc.2008.02.030
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Item Type: | Article |
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Item Status: | Live Archive |
Abstract
In rat hepatocytes, atrial natriuretic peptide (ANP) elevates cGMP through activation of particulate guanylyl cyclase and attenuates Ca2+ signals by stimulating net plasma membrane Ca2+ efflux. We show here that ANP-stimulated hepatocyte Ca2+ efflux is mediated by protein kinase G (PKG) isotype I. Furthermore, we show that ANP recruits endogenous PKGIα, but not PKGIβ, to the plasma membrane. These effects are mimicked by 8-bromo-cGMP, but not by the soluble guanylyl cyclase activators, sodium nitroprusside and YC-1. We propose that ANP, through localized cGMP elevation, promotes plasma membrane recruitment of PKGIα, which, in turn, stimulates Ca2+ efflux.
Keywords: | Atrial natriuretic peptide (ANP), Ca2+ efflux, cGMP compartmentation, cGMP-dependent protein kinase (cGK), Plasma membrane Ca2+-ATPase (PMCA), Protein kinase G (PKG), Hepatocytes |
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Subjects: | Library of Congress Subject Areas > Q Science > QH Natural history > QH301 Biology Library of Congress Subject Areas > Q Science > QP Physiology |
Divisions: | College of Science > School of Life Sciences |
ID Code: | 14076 |
Deposited On: | 30 Aug 2011 13:12 |
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