Protective effects of cysteine analogues on acute myocardial ischemia: novel modulators of endogenous H2S production

Wang, Qian and Wang, Xian-Li and Liu, Hong-Rui and Rose, Peter and Zhu, Yi-Zhun (2010) Protective effects of cysteine analogues on acute myocardial ischemia: novel modulators of endogenous H2S production. Antioxidants & Redox Signaling, 12 (10). pp. 1155-1165. ISSN 1523-0864

Full content URL: http://dx.doi.org/10.1089/ars.2009.2947

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Abstract

The current study was designed to evaluate the pharmacologic effects of three novel cysteine-containing compounds: S-propyl-l-cysteine (SPC), S-allyl-l-cysteine (SAC), and S-propargyl-l-cysteine (SPRC) on H(2)S production and antioxidant defenses in an acute myocardial infarction (MI) rat model. The enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as glutathione redox status and malonaldehyde (MDA) content, also were determined. All three compounds were found to preserve SOD and GPx activities and also tissue GSH levels while reducing the formation of the lipid peroxidation product MDA in ventricular tissues. With immunfluorescence assays, we observed the expression of CSE and Mn-SOD. The morphologic changes of the cardiac cells are seen with both light and electron microscopy. The corresponding pathologic alterations were characterized mainly as loss of adherence between cardiac myocytes and swollen or ruptured mitochondria at the ultrastructural level. Propargylglycine, a selective inhibitor of CSE, abolished the protective effects of each compound used in the current model. Our study provides novel evidence that SPC, SAC, and SPRC have cardioprotective effects in MI by reducing the deleterious effects of oxidative stress by modulating the endogenous levels of H(2)S and preserving the activities of antioxidant defensive enzymes like SOD.

Additional Information:Online Ahead of Print: February 26, 2010, Online Ahead of Editing: October 20, 2009
Keywords:Myocardial ischemia, catalase, cystathionine gamma lyase, cysteine derivative, glutathione, glutathione peroxidase, hydrogen sulfide, malonaldehyde, propargylglycine, s allylcysteine, s propargylcysteine, s propylcysteine, superoxide dismutase, unclassified drug, zyz 802, acute heart infarction, animal experiment, animal model, antioxidant activity, article, cell structure, controlled study, drug structure, drug synthesis, electron microscopy, enzyme activity, heart muscle cell, heart muscle ischemia, heart protection, heart ventricle, immunofluorescence test, lipid peroxidation, male, microscopy, mitochondrion, nonhuman, oxidation reduction reaction, oxidative stress, priority journal, protein expression, rat, Air Pollutants, Animals, Antioxidants, Cardiotonic Agents, Cysteine, Humans, Malondialdehyde, Myocardium, Oxidation-Reduction, Rats, Sprague-Dawley, RNA, Messenger, Rattus
Subjects:B Subjects allied to Medicine > B100 Anatomy, Physiology and Pathology
Divisions:College of Science > School of Life Sciences
ID Code:9804
Deposited On:10 Jun 2013 11:21

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