Improved method for i.r. determination of the degree of N-acetylation of chitosan

Baxter, Alasdair and Dillon, Michael and Taylor, K. D. Anthony and Roberts, George A. F. (1992) Improved method for i.r. determination of the degree of N-acetylation of chitosan. International Journal of Biological Macromolecules, 14 (3). pp. 166-169. ISSN 0141-8130

Full content URL: http://dx.doi.org/10.1016/S0141-8130(05)80007-8

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Improved method for i.r. determination of the degree of N-acetylation of chitosan
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Abstract

Three published i.r. absorption band ratios for determining the % N-acetylation of chitosan have been used to follow the rate of alkaline deacetylation of chitin. Only one, the A1655/A3450 ratio, gives the expected rate of deacetylation curve. Consideration of the various i.r. ratios has led to a new relationship, % N-acetylation = (A1655/A3450) × 115, where the absorbance of the amide I band at approximately 1655 cm−1 is determined using the baseline proposed previously. The values obtained using this proposed relationship agree closely with those obtained from dye adsorption measurements for chitosans having % N-acetylation values in the range 0–55%.

Keywords:Chitin, chitosan, N-acetylation, i.r. spectroscopy
Subjects:F Physical Sciences > F180 Analytical Chemistry
Divisions:College of Science > National Centre for Food Manufacturing
ID Code:9556
Deposited On:11 Jul 2013 15:04

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