Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction

Bruce, K. D. and Hiorns, W. D. and Hobman, J. L. and Osborn, A. M. and Strike, P. and Ritchie, D. A. (1992) Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction. Applied and Environmental Microbiology, 58 (10). pp. 3413-3416. ISSN 0099-2240

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Item Type:Article
Item Status:Live Archive

Abstract

Specific DNA sequences from native bacterial populations present in soil, sediment, and sand samples were amplified by using the polymerase chain reaction with primers for either 'universal' eubacterial 16S rRNA genes or mercury resistance (mer) genes. With standard amplification conditions, 1.5- kb rDNA fragments from all 12 samples examined and from as little as 5 μg of soil were reproducibly amplified. A 1-kb mer fragment from one soil sample was also amplified. The identity of these amplified fragments was confirmed by DNA-DNA hybridization.

Keywords:DNA fragment, bacterial genetics, bacterium colony, DNA DNA hybridization, DNA sequence, gene amplification, note, polymerase chain reaction, priority journal, reproducibility, sampling, soil microflora, Base Sequence, DNA, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, Soil Microbiology, Support, Non-U.S. Gov't, Bacteria (microorganisms)
Subjects:C Biological Sciences > C180 Ecology
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:8980
Deposited On:22 Apr 2013 11:56

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