Sequence conservation between regulatory mercury resistance genes in bacteria from mercury polluted and pristine environments

Osborn, A. M. and Bruce, K. D. and Strike, P. and Ritchie, D. A. (1995) Sequence conservation between regulatory mercury resistance genes in bacteria from mercury polluted and pristine environments. Systematic and Applied Microbiology, 18 (1). pp. 1-6. ISSN 0723-2020

Full text not available from this repository.

Item Type:Article
Item Status:Live Archive

Abstract

The regulatory gene merR, and the adjacent operator/promoter region was amplified from the mercury resistance (Hg(R)) determinants from 10 Gram-negative bacterial isolates from mercury polluted and pristine environments using the polymerase chain reaction. These mer regions showed polymorphism in size of PCR amplification products with those from isolates SE3, SE11, SE12, SE31, SO1 and T217 being of 557 base pairs in size, whilst those from isolates SE20, T238, SB3, SB4 and the positive control (Tn501) were 536 base pairs in size. From the sequence analysis of these mer regions and comparison with previously sequenced Hg(R) determinants an evolutionary tree was constructed which showed there to be a significant difference between Gram-negative merR genes and those found in Gram-positive organisms. With the exception of the Thiobacillus Hg(R) determinants, merR genes from Gram negative bacteria were strongly conserved and could be grouped closely around the previously sequenced determinants of Tn501, Tn21, Tn5053 (pMER327/419) and pKLH2. Only the merR genes of pDU1358 and T238 showed significant variation from these subgroups. The regions of greatest variation were the carboxyl terminal coding region of the merR gene and the operator/promoter region. It is suggested that, due to the global nature of inducible mercury resistance and its strong sequence conservation across large geographical distances, bacterial resistance to mercury is an ancient genetic character.

Keywords:mercury, antibiotic resistance, article, carboxy terminal sequence, genetic conservation, gram negative bacterium, gram positive bacterium, nonhuman, nucleotide sequence, operator gene, pollution, polymerase chain reaction, priority journal, promoter region, regulator gene
Subjects:C Biological Sciences > C180 Ecology
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
Related URLs:
ID Code:8977
Deposited On:31 May 2013 15:07

Repository Staff Only: item control page