Characterization of antibiotic resistance plasmids from Bordetella bronchiseptica

Speakman, A. J. and Binns, S. H. and Osborn, Mark and Corkill, J. E. and Kariuki, S. and Saunders, J. R. and Dawson, S. and Gaskell, R. M. and Hart, C. A. (1997) Characterization of antibiotic resistance plasmids from Bordetella bronchiseptica. Journal of Antimicrobial Chemotherapy, 40 (6). pp. 811-816. ISSN 0305-7453

Full content URL: http://jac.oxfordjournals.org/content/40/6/811

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Item Type:Article
Item Status:Live Archive

Abstract

Of 52 antibiotic-resistant Bordetella bronchiseptica isolates from cats, ten carried plasmids. Only two of these plasmids, pLV1400 and pLV1401, were self-transmissible to Escherichia coli K12; both plasmids encoded resistance to ampicillin, tetracycline, sulphonamides, streptomycin and mercuric chloride, and were of incompatibility group P (IncP). Transferable tetracycline resistance has not been reported in B. bronchiseptica previously. The plasmids were identical in size (c.51 kb), restriction endonuclease digestion pattern and gene sequences (trfA and korA) within the IncP replicon. The trfA and korA sequences differed from those of the archetypal IncP plasmids RP4 and R751. Although the two B. bronchiseptica isolates were from epidemiologically and geographically separated cats, pulsed-field gel electrophoresis of their XbaI- or DraI-digested chromosomal DNA indicated that they were genotypically identical. The plasmid-encoded ampicillin resistance was mediated by a penicillinase of molecular weight 49,000, and pI 8.45 which was inhibited by clavulanate (IC50 = 0.1 mg/L) and tazobactam (IC50 = 0.42 mg/L) but not by parachloromercuribenzoate or EDTA. The high-level tetracycline resistance was mediated by a class C efflux mechanism that has not been described previously in this genus. The presence of transferable multidrug resistance on a promiscuous plasmid may limit options for therapy of respiratory tract infection in companion and farm animals.

Keywords:amoxicillin, ampicillin, chlorobenzoic acid derivative, clavulanic acid, cloxacillin, doxycycline, edetic acid, enrofloxacin, mercuric chloride, penicillinase, restriction endonuclease, streptomycin, sulfadiazine, sulfonamide, tazobactam, tetracycline, trimethoprim, antibiotic resistance, antibiotic sensitivity, article, bordetella bronchiseptica, controlled study, drug potency, enzyme inhibition, escherichia coli, gene sequence, isoelectric point, minimum inhibitory concentration, molecular weight, multidrug resistance, nonhuman, plasmid, Animals, Anti-Bacterial Agents, Cats, Microbial Sensitivity Tests, R Factors, Tetracycline Resistance
Subjects:D Veterinary Sciences, Agriculture and related subjects > D324 Animal Pharmacology
D Veterinary Sciences, Agriculture and related subjects > D320 Animal Health
D Veterinary Sciences, Agriculture and related subjects > D300 Animal Science
Divisions:College of Science > School of Life Sciences
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ID Code:8972
Deposited On:12 Jun 2013 13:41

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