Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments

Osborn, A. Mark and Pickup, Roger W. and Saunders, Jon R. (2000) Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments. FEMS Microbiology Letters, 186 (2). pp. 203-208. ISSN 0378-1097

Full content URL: http://dx.doi.org/10.1016/S0378-1097(00)00147-6

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Item Type:Article
Item Status:Live Archive

Abstract

Homology to IncN, P, Q and W inc regions was investigated amongst 114 Hg2+-resistant or antibiotic-resistant bacteria isolated from lakewater sediments. No hybridisation signals were found with Inc P, Q and W probes, and only one plasmid, pLV1402, hybridised to the IncN probe. PCR primers designed to conserved regions in the replicon of the IncN plasmid pCU1 and the related beta replicon from pGSH500 were used to amplify a 978-bp fragment from pLV1402, with sequence analysis showing a close relationship (99.2 identity) between their replication genes. A 387-bp region from the pLV1402 rep gene was used to re-screen the isolates and identified another related plasmid, pLV1403. A 3.7-kb probe containing the alpha replicon from pGSH500 hybridised to both pLV1402 and pLV1403, suggesting that both are multi-replicon plasmids. The PCR primers and probes described will be useful in future studies of plasmid diversity. Copyright (C) 2000 Federation of European Microbiological Societies.

Keywords:bacterial DNA, lake water, mercury, antibiotic resistance, article, bacterial genetics, bacterium isolate, controlled study, Escherichia coli, gene amplification, molecular biology, nonhuman, nucleotide sequence, plasmid, polymerase chain reaction, priority journal, replicon, sediment, sequence analysis, sequence homology, technique, Bacteria, Cloning, Molecular, Conserved Sequence, DNA Primers, Drug Resistance, Microbial, Fresh Water, Genetic Techniques, Geologic Sediments, Plasmids
Subjects:C Biological Sciences > C180 Ecology
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
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ID Code:8969
Deposited On:06 May 2013 19:12

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