Diversity and linkage of replication and mobilisation genes in Bacillus rolling circle-replicating plasmids from diverse geographical origins

Mason, Vince P. and Syrett, Natalie and Hassanali, Tasneem and Osborn, A. Mark (2002) Diversity and linkage of replication and mobilisation genes in Bacillus rolling circle-replicating plasmids from diverse geographical origins. FEMS Microbiology Ecology, 42 (2). pp. 235-241. ISSN 0168-6496

Full content URL: http://dx.doi.org/10.1016/S0168-6496(02)00341-0

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Item Type:Article
Item Status:Live Archive

Abstract

The prevalence, diversity and linkage of pTA-related plasmid rep and mob genes in 18 plasmids from three closely related species of Bacillus from diverse geographical locations have been investigated using PCR. pTA-related rep and mob sequences were both amplified from 13 of the 18 plasmids. For one plasmid, pBM9, only a pTA-related mob gene was amplified, whilst only a pTA-related rep gene was amplified from the Bacillus licheniformis plasmid pBL2. No products were amplified for either gene from two other B. licheniformis plasmids or from a larger 16-kb Bacillus subtilis plasmid, pBS6. Whilst simple gene detection suggests close linkage of both pTA-related rep and mob genes, on most of these plasmids, sequence analysis of amplified genes revealed more complex linkage relationships varying with geographical origin, plasmid size and bacterial host. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Keywords:DNA, plasmid, article, Bacillus licheniformis, Bacillus subtilis, controlled study, gene amplification, gene replication, gene sequence, genetic linkage, genetic variability, nonhuman, nucleotide sequence, plasmid, polymerase chain reaction, priority journal, sequence analysis, Bacillus licheniformis, Bacteria (microorganisms)
Subjects:C Biological Sciences > C180 Ecology
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
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ID Code:8963
Deposited On:06 May 2013 18:37

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