Determining the identity and roles of oil-metabolizing marine bacteria from the Thames estuary, UK

McKew, B. A. and Coulon, F. and Osborn, A. M. and Timmis, K. N. and McGenity, T. J. (2007) Determining the identity and roles of oil-metabolizing marine bacteria from the Thames estuary, UK. Environmental Microbiology, 9 (1). pp. 165-176. ISSN 1462-2912

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Item Type:Article
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Abstract

Crude oil is a complex mixture of different hydrocarbons. While diverse bacterial communities can degrade oil, the specific roles of individual members within such communities remain unclear. To identify the key bacterial taxa involved in aerobic degradation of specific hydrocarbons, microcosm experiments were established using seawater from Stanford le Hope, Thames estuary, UK, adjacent to a major oil refinery. In all microcosms, hydrocarbon degradation was significant within 10 weeks, ranging from > 99 of low-molecular-weight alkanes (C10-C18), 41-84 of high-molecular-weight alkanes (C20-C32) and pristane, and 32-88 of polycyclic aromatic hydrocarbons (PAHs). Analysis of 16S rRNA sequences from clone libraries and denaturing gradient gel electrophoresis (DGGE) indicated that, except when incubated with fluorene, PAH-degrading communities were dominated by Cycloclasticus. Moreover, PAH-degrading communities were distinct from those in microcosms containing alkanes. Degradation of the branched alkane, pristane, was carried out almost exclusively by Alcanivorax. Bacteria related to Thalassolituus oleivorans (99-100 identity) were the dominant known alkane degraders in n-alkane (C12-C32) microcosms, while Roseobacter-related bacteria were also consistently found in these microcosms. However, in contrast to previous studies, Thalassolituus, rather than Alcanivorax, was dominant in crude oil-enriched microcosms. The communities in n-decane microcosms differed from those in microcosms supplemented with less volatile alkanes, with a phylogenetically distinct species of Thalassolituus out-competing T. oleivorans. These data suggest that the diversity and importance of the genus Thalassolituus is greater than previously established. Overall, these experiments demonstrate how degradation of different petroleum hydrocarbons is partitioned between different bacterial taxa, which together as a community can remediate petroleum hydrocarbon-impacted estuarine environments. © 2006 The Authors.

Keywords:aromatic hydrocarbon, petroleum, ribosome DNA, RNA 16S, sea water, article, bioremediation, classification, ecosystem, electrophoresis, genetics, isolation and purification, metabolism, methodology, microbiology, phylogeny, Proteobacteria, reverse transcription polymerase chain reaction, temperature, United Kingdom, Biodegradation, Environmental, DNA, Ribosomal, Great Britain, Hydrocarbons, Aromatic, Reverse Transcriptase Polymerase Chain Reaction, RNA, Ribosomal, 16S, Seawater, Temperature, Alcanivorax, Bacteria (microorganisms), Cycloclasticus, Roseobacter, Thalassolituus, Thalassolituus oleivorans
Subjects:C Biological Sciences > C180 Ecology
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:8954
Deposited On:22 Apr 2013 09:56

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