Using a large area CMOS APS for direct chemiluminescence detection in western blotting electrophoresis

Esposito, Michela and Newcombe, Jane and Anaxagoras, Thalis and Allinson, Nigel M. and Wells, Kevin (2012) Using a large area CMOS APS for direct chemiluminescence detection in western blotting electrophoresis. In: Conference of Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging, 5-7 February 2012, San Diego, California.

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Item Type:Conference or Workshop contribution (Paper)
Item Status:Live Archive

Abstract

Western blotting electrophoretic sequencing is an analytical technique widely used in Functional Proteomics to detect, recognize and quantify specific labelled proteins in biological samples. A commonly used label for western blotting is Enhanced ChemiLuminescence (ECL) reagents based on fluorescent light emission of Luminol at 425nm. Film emulsion is the conventional detection medium, but is characterized by non-linear response and limited dynamic range. Several western blotting digital imaging systems have being developed, mainly based on the use of cooled Charge Coupled Devices (CCDs) and single avalanche diodes that address these issues. Even so these systems present key drawbacks, such as a low frame rate and require operation at low temperature. Direct optical detection using Complementary Metal Oxide Semiconductor (CMOS) Active Pixel Sensors (APS) could represent a suitable digital alternative for this application. In this paper the authors demonstrate the viability of direct chemiluminescent light detection in western blotting electrophoresis using a CMOS APS at room temperature. Furthermore, in recent years, improvements in fabrication techniques have made available reliable processes for very large imagers, which can be now scaled up to wafer size, allowing direct contact imaging of full size western blotting samples. We propose using a novel wafer scale APS (12.8 cm�13.2 cm), with an array architecture using two different pixel geometries that can deliver an inherently low noise and high dynamic range image at the same time representing a dramatic improvement with respect to the current western blotting imaging systems. © 2012 SPIE.

Additional Information:Conference code 89690
Keywords:APS, CMOS, Protein detection, Room temperature, Western blotting, Charge coupled devices, Chemiluminescence, Electrophoresis, Emulsification, Imaging systems, Medical applications, Molecular biology, Proteins, Molecular imaging
Subjects:G Mathematical and Computer Sciences > G900 Others in Mathematical and Computing Sciences
Divisions:College of Science > School of Computer Science
ID Code:8530
Deposited On:07 Apr 2013 19:48

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