Wave front engineering for microscopy of living cells

Emiliani, Valentina and Cojoc, Dan and Ferrari, Enrico and Garbin, Valeria and Durieux, Christiane and Coppey-Moisan, Maite and Di Fabrizio, Enzo (2005) Wave front engineering for microscopy of living cells. Optics Express, 13 (5). pp. 1395-1405. ISSN 1094-4087

Full content URL: http://www.opticsinfobase.org/oe/abstract.cfm?URI=...

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Item Type:Article
Item Status:Live Archive

Abstract

A new method to perform simultaneously three dimensional optical sectioning and optical manipulation is presented. The system combines a multi trap optical tweezers with a video microscope to enable axial scanning of living cells while maintaining the trapping configuration at a fixed position. This is achieved compensating the axial movement of the objective by shaping the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. Our method has been validated in three different experimental configurations. In the first, we decouple the position of a trapping plane from the axial movements of the objective and perform optical sectioning of a circle of beads kept on a fixed plane. In a second experiment, we extend the method to living cell microscopy by showing that mechanical constraints can be applied on the dorsal surface of a cell whilst performing its fluorescence optical sectioning. In the third experiment, we trapped beads in a three dimensional geometry and perform, always through the same objective, an axial scan of the volume delimited by the beads.

Keywords:optical manipulation, holographic optical tweezers, Spatial light modulators
Subjects:F Physical Sciences > F360 Optical Physics
F Physical Sciences > F310 Applied Physics
F Physical Sciences > F361 Laser Physics
Divisions:College of Science > School of Life Sciences
ID Code:8385
Deposited On:30 Mar 2013 22:14

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