High-performance liquid chromatography accelerator mass spectrometry: Correcting for losses during analysis by internal standardization

Lappin, Graham and Simpson, M. and Shishikura, Y. and Garner, C. (2008) High-performance liquid chromatography accelerator mass spectrometry: Correcting for losses during analysis by internal standardization. Analytical Biochemistry, 378 (1). pp. 93-95. ISSN 0003-2697

Full content URL: http://dx.doi.org/10.1016/j.ab.2008.03.026

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Item Type:Article
Item Status:Live Archive

Abstract

A method was developed to account for analytical losses of 14C-analyte when determining the concentration in biological samples using chromatographic separation and analysis by accelerator mass spectrometry. From the equations of J. Vogel and A.H. Love (in: A.L. Burlingame (Ed.), Methods in Enzymology, Academic Press, New York, 2005), new equations were derived to describe the isotopic dilution of a chromatographically isolated 14C-analyte. The analytical recovery for each sample was determined by the use of the UV response for nonlabeled analyte, as an internal standard against a standard curve. The slope of the curve was substituted into the equations to provide a method of accurately determining the analyte concentration. © 2008 Elsevier Inc. All rights reserved.

Keywords:carbon 14, accelerator mass spectrometry, analytic method, article, diagnostic accuracy, dilution, high performance liquid chromatography, mass spectrometry, mathematical computing, priority journal, separation technique, standardization, ultraviolet radiation, Chromatography, High Pressure Liquid
Divisions:College of Science > School of Pharmacy
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ID Code:8226
Deposited On:24 Mar 2013 21:16

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