Development of 2D chiral chromatography with accelerator mass spectrometry for quantification of 14C-labeled R- and S-verapamil in plasma

Simpson, M. and Lappin, G. and Keely, B. J. (2010) Development of 2D chiral chromatography with accelerator mass spectrometry for quantification of 14C-labeled R- and S-verapamil in plasma. Bioanalysis, 2 (3). pp. 397-405. ISSN 1757-6180

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Abstract

Background: A microdose study was performed where 50 μg R/S- 14C-verapamil was dosed intravenously to human volunteers. In order to quantify the individual R- and S-enantiomers in human plasma a 2D chiral HPLC method with subsequent analysis by accelerator mass spectrometry was verified. Results: R/S-verapamil was separated on a C18 column and the isolated fraction was applied to a chiral column where the verapamil enantiomers were separated. Experimental recovery (�73 coefficient of variation {CV} = 16% and 66% CV = 21% for R- and S-verapamil, respectively) was accounted for by the use of internal standardization from the fluorescence response of nonlabeled R- and S-verapamil. The precision of the assay ranged from 4.1 to 15.9% CV and the limit of quantitation was 1.95-4.81 pg/ml for R-verapamil and 1.76-3.34 pg/ml for S-verapamil. Conclusion: This method was successfully applied to the analysis of R- and S-verapamil in human plasma. © 2010 Future Science Ltd.

Keywords:carbon 14, verapamil, carbon, verapamil, accelerator mass spectrometry, accuracy, article, chiral chromatography, controlled study, drug blood level, drug determination, drug microdose, drug structure, enantiomer, fluorescence, high performance liquid chromatography, human, human experiment, isotope labeling, limit of quantitation, male, mass spectrometry, normal human, standardization, blood, blood analysis, chemistry, isolation and purification, methodology, phase 1 clinical trial (topic), stereoisomerism, Blood Chemical Analysis, Carbon Radioisotopes, Chromatography, High Pressure Liquid, Clinical Trials, Phase I as Topic, Humans, Stereoisomerism
Divisions:College of Science > School of Pharmacy
ID Code:8216
Deposited On:21 Mar 2013 17:11

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