An assessment of the thermal stability of Clostridium difficile toxoid formulations

Lancaster, Lorna and Markey, Kevin and Ho, Mei (2011) An assessment of the thermal stability of Clostridium difficile toxoid formulations. Human Vaccines, 7 (2). pp. 202-210. ISSN 1554-8600

Full content URL: http://dx.doi.org/10.4161/hv.7.2.13986

Full text not available from this repository.

Item Type:Article
Item Status:Live Archive

Abstract

Strains of Clostridium difficile produce toxins A and B that can cause diarrhoea and pseudomembranous colitis. Currently, there is no preventative therapy for this infection but antibodies to the toxins provide protection, therefore a toxoid-based vaccine is needed. To evaluate thermal stability, a lyophilized and liquid formulation of toxoids A and B were stored at a range of temperatures for 5 weeks. Changes in toxoid structures and immune responses in an animal model before and after the incubation period were assessed. The structural integrity and the immune responses to liquid formulations were affected when stored at 56°C but the lyophilized formulation was thermally stable and same treatment did not result in significant loss of immunological responses when immunized in an animal model.

Additional Information:Strains of Clostridium difficile produce toxins A and B that can cause diarrhoea and pseudomembranous colitis. Currently, there is no preventative therapy for this infection but antibodies to the toxins provide protection, therefore a toxoid-based vaccine is needed. To evaluate thermal stability, a lyophilized and liquid formulation of toxoids A and B were stored at a range of temperatures for 5 weeks. Changes in toxoid structures and immune responses in an animal model before and after the incubation period were assessed. The structural integrity and the immune responses to liquid formulations were affected when stored at 56°C but the lyophilized formulation was thermally stable and same treatment did not result in significant loss of immunological responses when immunized in an animal model.
Keywords:Vaccine, Clostridium difficile, toxoids, nosocomial, thermal stability
Subjects:C Biological Sciences > C550 Immunology
C Biological Sciences > C710 Applied Molecular Biology, Biophysics and Biochemistry
C Biological Sciences > C521 Medical Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:7512
Deposited On:12 Feb 2013 16:43

Repository Staff Only: item control page