A computational approach for peptidomic analysis in taxonomic study of amphibian species

Zheng, H. and Ojha, P. C. and McClean, S. and Graham, C. and Black, N. D. and Hughes, J. G. and Shaw, C. (2005) A computational approach for peptidomic analysis in taxonomic study of amphibian species. IEEE Trans Nanobioscience, 4 (3). pp. 241-247. ISSN 1536-1241

Full content URL: http://dx.doi.org/10.1109/TNB.2005.853663

Full text not available from this repository.

Item Type:Article
Item Status:Live Archive

Abstract

Peptides in the skin secretion of frogs have been studied for some time now because they frequently possess important biological activity such as antibiotic, antimicrobial, or anticancer properties. In this paper, we present a computational approach for measuring the degree of similarity between the entire peptide complement of the skin secretion of specimens from the same or different species. The first step in the analysis is the generation of a mass spectral profile from an experimental high-performance liquid chromatography/electrosparay ionization analysis of the sample. An "overlap" between the mass spectral profiles of different specimens is then proposed as a measure of their similarity. Analysis of specimens from three species of the genus Litoria, viz., L. Aurea, L. Caerulea, and L. Infrafrenata, and Rana Capito of genus Rana shows that the degree of similarity is highest between specimens from the same species, lower for specimens from different species of the same genus, and lowest between specimens from different genera. This indicates that comparison of skin peptide profiles (i.e., mass spectral profiles of skin secretion) is potentially a useful aid in the taxonomic study of amphibian species.

Additional Information:Peptides in the skin secretion of frogs have been studied for some time now because they frequently possess important biological activity such as antibiotic, antimicrobial, or anticancer properties. In this paper, we present a computational approach for measuring the degree of similarity between the entire peptide complement of the skin secretion of specimens from the same or different species. The first step in the analysis is the generation of a mass spectral profile from an experimental high-performance liquid chromatography/electrosparay ionization analysis of the sample. An "overlap" between the mass spectral profiles of different specimens is then proposed as a measure of their similarity. Analysis of specimens from three species of the genus Litoria, viz., L. Aurea, L. Caerulea, and L. Infrafrenata, and Rana Capito of genus Rana shows that the degree of similarity is highest between specimens from the same species, lower for specimens from different species of the same genus, and lowest between specimens from different genera. This indicates that comparison of skin peptide profiles (i.e., mass spectral profiles of skin secretion) is potentially a useful aid in the taxonomic study of amphibian species.
Keywords:Mass Spectrometry, Skin Peptide Profiles, Rana Capito, Anuran
Subjects:C Biological Sciences > C560 Biotechnology
Divisions:College of Science > School of Life Sciences
ID Code:7336
Deposited On:22 Jan 2013 15:58

Repository Staff Only: item control page