Optical tweezers for sample fixing in micro‐diffraction experiments

Amenitsch, H. and Cojoc, D. and Rappolt, M. and Sartori, B. and Laggner, P. and Ferrari, Enrico and Garbin, V. and Burghammer, M. and Riekel, C. and Di Fabrizio, E. (2006) Optical tweezers for sample fixing in micro‐diffraction experiments. In: Synchrotron Radiation Instrumentation: Ninth International Conference on Synchrotron Radiation Instrumentation, 8 May-2 June 2006, Daegu, Korea.

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Official URL: http://proceedings.aip.org/resource/2/apcpcs/879/1...

Abstract

In order to manipulate, characterize and measure the micro‐diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi‐lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X‐ray diffraction.

Item Type:Conference or Workshop Item (Paper)
Additional Information:In order to manipulate, characterize and measure the micro‐diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi‐lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X‐ray diffraction.
Keywords:X-ray diffraction, biomedical imaging, lipid bilayers
Subjects:F Physical Sciences > F310 Applied Physics
F Physical Sciences > F361 Laser Physics
Divisions:College of Science > School of Life Sciences
ID Code:6982
Deposited By: Enrico Ferrari
Deposited On:27 Nov 2012 23:38
Last Modified:27 Nov 2012 23:38

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