Gates, Andrew J. and Luque-Almagro, Victor M. and Goddard, Alan D. and Ferguson, Stuart J. and Roldán, M. Dolores and Richardson, David J. (2011) A composite biochemical system for bacterial nitrate and nitrite assimilation as exemplified by Paracoccus denitrificans. Biochemical Journal, 435 (3). pp. 743-753. ISSN 0264-6021
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Official URL: http://dx.doi.org/10.1042/BJ20101920
The denitrifying bacterium Paracoccus denitrificans can grow aerobically or anaerobically using nitrate or nitrite as the sole nitrogen source. The biochemical pathway responsible is expressed from a gene cluster comprising a nitrate/nitrite transporter (NasA), nitrite transporter (NasH), nitrite reductase (NasB), ferredoxin (NasG) and nitrate reductase (NasC). NasB and NasG are essential for growth with nitrate or nitrite as the nitrogen source. NADH serves as the electron donor for nitrate and nitrite reduction, but only NasB has a NADH-oxidizing domain. Nitrate and nitrite reductase activities show the same K m for NADH and can be separated by anion-exchange chromatography, but only fractions containing NasB retain the ability to oxidize NADH. This implies that NasG mediates electron flux from the NADH-oxidizing site in NasB to the sites of nitrate and nitrite reduction in NasC and NasB respectively. Delivery of extracellular nitrate to NasBGC is mediated by NasA, but both NasA and NasH contribute to nitrite uptake. The roles of NasA and NasC can be substituted during anaerobic growth by the biochemically distinct membrane-bound respiratory nitrate reductase (Nar), demonstrating functional overlap. nasG is highly conserved in nitrate/nitrite assimilation gene clusters, which is consistent with a key role for the NasG ferredoxin, as part of a phylogenetically widespread composite nitrate and nitrite reductase system.
|Keywords:||Nitrate reductase, Nitrate transport, Nitrite reductase, Nitrogen assimilation|
|Subjects:||C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry|
|Divisions:||College of Science > School of Life Sciences|
|Deposited By:||Alan Goddard|
|Deposited On:||16 Nov 2012 09:36|
|Last Modified:||29 Nov 2013 09:45|
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