DNA-templated protein arrays for single-molecule imaging

Selmi, Daniele N. and Adamson, Roslin J. and Attrill, Helen and Goddard, Alan D. and Gilbert, Robert J. C. and Watts , Anthony and Turberfield, Andrew J. (2011) DNA-templated protein arrays for single-molecule imaging. Nano Letters, 11 (2). pp. 657-660. ISSN 1530-6984

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Official URL: http://dx.doi.org/10.1021/nl1037769

Abstract

Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

Item Type:Article
Keywords:DNA templates, G-protein-coupled membrane receptor, nanoaffinity, nanostructure, Single-particle electron cryomicrosopy
Subjects:C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry
Divisions:College of Science > School of Life Sciences
ID Code:6839
Deposited By: Alan Goddard
Deposited On:16 Nov 2012 09:20
Last Modified:29 Nov 2013 09:41

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