Selmi, Daniele N. and Adamson, Roslin J. and Attrill, Helen and Goddard, Alan D. and Gilbert, Robert J. C. and Watts , Anthony and Turberfield, Andrew J. (2011) DNA-templated protein arrays for single-molecule imaging. Nano Letters, 11 (2). pp. 657-660. ISSN 1530-6984
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Official URL: http://dx.doi.org/10.1021/nl1037769
Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.
|Keywords:||DNA templates, G-protein-coupled membrane receptor, nanoaffinity, nanostructure, Single-particle electron cryomicrosopy|
|Subjects:||C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry|
|Divisions:||College of Science > School of Life Sciences|
|Deposited By:||Alan Goddard|
|Deposited On:||16 Nov 2012 09:20|
|Last Modified:||29 Nov 2013 09:41|
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