Goddard, Alan and Stevens, Julie M. and Rao, Feng and Mavridou, Despoina A. I. and Chan, Weelee and Richardson, David J. and Allen, James W. A. and Ferguson, Stuart J. (2010) c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. Journal of Biological Chemistry, 285 (30). pp. 22882-22889. ISSN 0021-9258
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Official URL: http://dx.doi.org/10.1074/jbc.M110.133421
The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.
|Subjects:||C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry|
|Divisions:||College of Science > School of Life Sciences|
|Deposited By:||Alan Goddard|
|Deposited On:||16 Nov 2012 09:08|
|Last Modified:||29 Nov 2013 09:03|
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