Identification of Gnr1p, a negative regulator of Gα signalling in Schizosaccharomyces pombe, and its complementation by human Gβ subunits

Goddard, Alan and Ladds, Graham and Forfar, Rachel and Davey, John (2006) Identification of Gnr1p, a negative regulator of Gα signalling in Schizosaccharomyces pombe, and its complementation by human Gβ subunits. Fungal Genetics and Biology, 43 (12). pp. 840-851. ISSN 1087-1845

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Official URL: http://dx.doi.org/10.1016/j.fgb.2006.06.005

Abstract

G protein-coupled receptors (GPCRs) are involved in the response of eukaryotic cells to a wide variety of stimuli, traditionally mediating their effects through heterotrimeric G proteins comprised of Gα, Gβ and Gγ subunits. The fission yeast Schizosaccharomyces pombe is an established tool for GPCR research, possessing two Gα-dependent signalling cascades. A complete Gαβγ complex has been characterised for the glucose-sensing pathway, but only the Gα subunit, Gpa1p, has been identified in the pheromone-response pathway. Here, we report the use of the yeast two-hybrid system to identify a novel protein, Gnr1p, which interacts with Gpa1p. Gnr1p is predicted to contain seven WD repeats and to adopt a structure similar to typical Gβ subunits. Disruption and overexpression studies reveal that Gnr1p negatively regulates the pheromone-response pathway but is not required for signalling. Human Gβ subunits complement the loss of Gnr1p, functioning as negative regulators of Gα signalling in fission yeast.

Item Type:Article
Additional Information:G protein-coupled receptors (GPCRs) are involved in the response of eukaryotic cells to a wide variety of stimuli, traditionally mediating their effects through heterotrimeric G proteins comprised of Gα, Gβ and Gγ subunits. The fission yeast Schizosaccharomyces pombe is an established tool for GPCR research, possessing two Gα-dependent signalling cascades. A complete Gαβγ complex has been characterised for the glucose-sensing pathway, but only the Gα subunit, Gpa1p, has been identified in the pheromone-response pathway. Here, we report the use of the yeast two-hybrid system to identify a novel protein, Gnr1p, which interacts with Gpa1p. Gnr1p is predicted to contain seven WD repeats and to adopt a structure similar to typical Gβ subunits. Disruption and overexpression studies reveal that Gnr1p negatively regulates the pheromone-response pathway but is not required for signalling. Human Gβ subunits complement the loss of Gnr1p, functioning as negative regulators of Gα signalling in fission yeast.
Keywords:Gβ subunit, Gnr1p, Gpa1p, Pheromone, Schizosaccharomyces pombe, WD repeat protein
Subjects:C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry
Divisions:College of Science > School of Life Sciences
ID Code:6828
Deposited By: Alan Goddard
Deposited On:15 Nov 2012 21:09
Last Modified:15 Nov 2012 21:09

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