Goddard, Alan and Ladds, Graham and Forfar, Rachel and Davey, John (2006) Identification of Gnr1p, a negative regulator of Gα signalling in Schizosaccharomyces pombe, and its complementation by human Gβ subunits. Fungal Genetics and Biology, 43 (12). pp. 840-851. ISSN 1087-1845
Full text not available from this repository.Abstract
G protein-coupled receptors (GPCRs) are involved in the response of eukaryotic cells to a wide variety of stimuli, traditionally mediating their effects through heterotrimeric G proteins comprised of Gα, Gβ and Gγ subunits. The fission yeast Schizosaccharomyces pombe is an established tool for GPCR research, possessing two Gα-dependent signalling cascades. A complete Gαβγ complex has been characterised for the glucose-sensing pathway, but only the Gα subunit, Gpa1p, has been identified in the pheromone-response pathway. Here, we report the use of the yeast two-hybrid system to identify a novel protein, Gnr1p, which interacts with Gpa1p. Gnr1p is predicted to contain seven WD repeats and to adopt a structure similar to typical Gβ subunits. Disruption and overexpression studies reveal that Gnr1p negatively regulates the pheromone-response pathway but is not required for signalling. Human Gβ subunits complement the loss of Gnr1p, functioning as negative regulators of Gα signalling in fission yeast.
| Item Type: | Article |
|---|---|
| Additional Information: | G protein-coupled receptors (GPCRs) are involved in the response of eukaryotic cells to a wide variety of stimuli, traditionally mediating their effects through heterotrimeric G proteins comprised of Gα, Gβ and Gγ subunits. The fission yeast Schizosaccharomyces pombe is an established tool for GPCR research, possessing two Gα-dependent signalling cascades. A complete Gαβγ complex has been characterised for the glucose-sensing pathway, but only the Gα subunit, Gpa1p, has been identified in the pheromone-response pathway. Here, we report the use of the yeast two-hybrid system to identify a novel protein, Gnr1p, which interacts with Gpa1p. Gnr1p is predicted to contain seven WD repeats and to adopt a structure similar to typical Gβ subunits. Disruption and overexpression studies reveal that Gnr1p negatively regulates the pheromone-response pathway but is not required for signalling. Human Gβ subunits complement the loss of Gnr1p, functioning as negative regulators of Gα signalling in fission yeast. |
| Keywords: | Gβ subunit, Gnr1p, Gpa1p, Pheromone, Schizosaccharomyces pombe, WD repeat protein |
| Subjects: | C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry |
| Divisions: | College of Sciences > Faculty of Science > School of Life Sciences |
| Depositing User: | Alan Goddard |
| Date Deposited: | 15 Nov 2012 21:09 |
| Last Modified: | 15 Nov 2012 21:09 |
| URI: | http://eprints.lincoln.ac.uk/id/eprint/6828 |
Actions (login required)
 |
View Item |