NanoLock polypeptide for the permanent immobilization of recombinant proteins on SNAP25 functionalized supports

Ferrari, Enrico and Darios, Frederic and Soloviev, Mikhail and Davletov, Bazbek (2010) NanoLock polypeptide for the permanent immobilization of recombinant proteins on SNAP25 functionalized supports. In: 3rd International NanoBio Conference, August 24-27 2010, Zurich, Switzerland.

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Full text URL: http://www.ecmjournal.org/journal/supplements/vol0...

Abstract

Many techniques in molecular biology, clinical diagnostics and biotechnology rely on binary affinity tags. The existing tags are based on either small molecules or peptide tags. Among these, the biotin-streptavidin system is most popular due to the nearly irreversible interaction of biotin with the tetrameric protein, streptavidin. The major drawback of the stable biotin-streptavidin system, however, is that neither of the two tags can be added to a protein of interest via recombinant means leading to the requirement for chemical coupling. Here we report a new immobilization system which utilizes two monomeric polypeptides which self-assemble to produce non-covalent yet nearly irreversible complex which is stable in strong detergents, chaotropic agents, as well as in acids and alkali.

Item Type:Conference or Workshop Item (Paper)
Additional Information:Many techniques in molecular biology, clinical diagnostics and biotechnology rely on binary affinity tags. The existing tags are based on either small molecules or peptide tags. Among these, the biotin-streptavidin system is most popular due to the nearly irreversible interaction of biotin with the tetrameric protein, streptavidin. The major drawback of the stable biotin-streptavidin system, however, is that neither of the two tags can be added to a protein of interest via recombinant means leading to the requirement for chemical coupling. Here we report a new immobilization system which utilizes two monomeric polypeptides which self-assemble to produce non-covalent yet nearly irreversible complex which is stable in strong detergents, chaotropic agents, as well as in acids and alkali.
Keywords:protein immobilization, SNAREs proteins
Subjects:C Biological Sciences > C710 Applied Molecular Biology, Biophysics and Biochemistry
Divisions:College of Science > School of Life Sciences
ID Code:6604
Deposited By: Enrico Ferrari
Deposited On:20 Oct 2012 07:58
Last Modified:20 Oct 2012 07:58

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