Ferrari, Enrico and Darios, Frederic and Soloviev, Mikhail and Davletov, Bazbek (2010) NanoLock polypeptide for the permanent immobilization of recombinant proteins on SNAP25 functionalized supports. In: 3rd International NanoBio Conference, August 24-27 2010, Zurich, Switzerland.
|Item Type:||Conference or Workshop Item (Paper)|
|Divisions:||College of Science > School of Life Sciences|
|Abstract:||Many techniques in molecular biology, clinical diagnostics and biotechnology rely on binary affinity tags. The existing tags are based on either small molecules or peptide tags. Among these, the biotin-streptavidin system is most popular due to the nearly irreversible interaction of biotin with the tetrameric protein, streptavidin. The major drawback of the stable biotin-streptavidin system, however, is that neither of the two tags can be added to a protein of interest via recombinant means leading to the requirement for chemical coupling. Here we report a new immobilization system which utilizes two monomeric polypeptides which self-assemble to produce non-covalent yet nearly irreversible complex which is stable in strong detergents, chaotropic agents, as well as in acids and alkali.|
|Date Deposited:||20 Oct 2012 07:58|
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