Local x-ray structure analysis of optically manipulated biological micro-objects

Cojoc, Dan and Amenitsch, Heinz and Ferrari, Enrico and Santucci, Silvia C. and Sartori, Barbara and Rappolt, Michael and Marmiroli, Benedetta and Burghammer, Manfred and Riekel, Christian (2010) Local x-ray structure analysis of optically manipulated biological micro-objects. Applied Physics Letters, 97 (24). p. 244101. ISSN 0003-6951

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Full text URL: http://dx.doi.org/10.1063/1.3525941

Abstract

X-ray diffraction using micro- and nanofocused beams is well suited for nanostructure analysis at different sites of a biological micro-object. To conduct in vitro studies without mechanical contact, we developed object manipulation by optical tweezers in a microfluidic cell. Here we report x-ray microdiffraction analysis of a micro-object optically trapped in three dimensions. We revealed the nanostructure of a single starch granule at different points and investigated local radiation damage induced by repeated x-ray exposures at the same position, demonstrating high stability and full control of the granule orientation by multiple optical traps.

Item Type:Article
Additional Information:X-ray diffraction using micro- and nanofocused beams is well suited for nanostructure analysis at different sites of a biological micro-object. To conduct in vitro studies without mechanical contact, we developed object manipulation by optical tweezers in a microfluidic cell. Here we report x-ray microdiffraction analysis of a micro-object optically trapped in three dimensions. We revealed the nanostructure of a single starch granule at different points and investigated local radiation damage induced by repeated x-ray exposures at the same position, demonstrating high stability and full control of the granule orientation by multiple optical traps.
Keywords:X-ray diffraction, optical tweezers
Subjects:F Physical Sciences > F360 Optical Physics
Divisions:College of Science > School of Life Sciences
ID Code:6584
Deposited By: Enrico Ferrari
Deposited On:15 Oct 2012 20:34
Last Modified:23 Jan 2013 16:38

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