Optimized multi-view imaging improves the observation of optically manipulated non-spherical particles

Selvaggi , Lara and Ferrari, Enrico and Moradi, Alireza and Santucci, Silvia C. and Beuzer, Paolo and Cojoc, Dan (2010) Optimized multi-view imaging improves the observation of optically manipulated non-spherical particles. Journal of Optics, 12 (3). 035303 . ISSN 2040-8978

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Official URL: http://dx.doi.org/10.1088/2040-8978/12/3/035303

Abstract

We report the optimization of two-side sample imaging through two orthogonal objectives and sample manipulation by single-beam optical trapping through one of the objectives. A high numerical aperture objective (NA = 1.0) is used to generate multiple laser traps configured by phase diffractive optical elements implemented on a phase programmable modulator. Supplementary lateral imaging through a NA = 0.55 objective represents a trade-off between the resolution and the field of view that provides additional information about the sample and its environment, impossible to obtain from conventional optical trapping setups where imaging is performed only along the trapping objective axis. The usefulness of two-side imaging combined with optical manipulation is illustrated by three different experiments: multiple beads trapped in 3D arrays, red blood cells rotated by multiple tweezers and manipulation of a glass grain with irregular shape.

Item Type:Article
Additional Information:We report the optimization of two-side sample imaging through two orthogonal objectives and sample manipulation by single-beam optical trapping through one of the objectives. A high numerical aperture objective (NA = 1.0) is used to generate multiple laser traps configured by phase diffractive optical elements implemented on a phase programmable modulator. Supplementary lateral imaging through a NA = 0.55 objective represents a trade-off between the resolution and the field of view that provides additional information about the sample and its environment, impossible to obtain from conventional optical trapping setups where imaging is performed only along the trapping objective axis. The usefulness of two-side imaging combined with optical manipulation is illustrated by three different experiments: multiple beads trapped in 3D arrays, red blood cells rotated by multiple tweezers and manipulation of a glass grain with irregular shape.
Keywords:orthogonal imaging, optical tweezers
Subjects:F Physical Sciences > F360 Optical Physics
Divisions:College of Science > School of Life Sciences
ID Code:6583
Deposited By: Enrico Ferrari
Deposited On:15 Oct 2012 20:39
Last Modified:02 Jan 2014 16:52

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