Adhesion through single peptide aptamers

Aubin-Tam, Marie-Eve and Appleyard, David C. and Ferrari, Enrico and Garbin, Valeria and Fadiran, Oluwatimilehin O. and Kunkel, Jacquelyn and Lang, Matthew J. (2011) Adhesion through single peptide aptamers. The Journal of Physical Chemistry A, 115 (16). pp. 3657-3664. ISSN 1089-5639

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Official URL: http://dx.doi.org/10.1021/jp1031493

Abstract

Aptamer and antibody mediated adhesion is central to biological function and is valuable in the engineering of “lab on a chip” devices. Single molecule force spectroscopy using optical tweezers enables direct nonequilibrium measurement of these noncovalent interactions for three peptide aptamers selected for glass, polystyrene, and carbon nanotubes. A comprehensive examination of the strong attachment between antifluorescein 4−4−20 and fluorescein was also carried out using the same assay. Bond lifetime, barrier width, and free energy of activation are extracted from unbinding histogram data using three single molecule pulling models. The evaluated aptamers appear to adhere stronger than the fluorescein antibody under no- and low-load conditions, yet weaker than antibodies at loads above 25 pN. Comparison to force spectroscopy data of other biological linkages shows the diversity of load dependent binding and provides insight into linkages used in biological processes and those designed for engineered systems.

Item Type:Article
Keywords:Aptamers, Force spectroscopy, Single molecule
Subjects:F Physical Sciences > F165 Biomolecular Chemistry
C Biological Sciences > C770 Biophysical Science
Divisions:College of Science > School of Life Sciences
ID Code:6579
Deposited By: Enrico Ferrari
Deposited On:15 Oct 2012 20:21
Last Modified:02 Sep 2013 13:56

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