Martinez-Fleites, Carlos and Tarbouriech, Nicolas and Ortiz-Lombardia, Miguel and Taylor, Edward and Rodriguez, Armando and Ramirez, Ricardo and Hernandez, Lazaro and Davies, Gideon J. (2004) Crystallization and preliminary X-ray diffraction analysis of levansucrase (LsdA) from Gluconacetobacter diazotrophicus SRT4. Acta Crystallographica Section D, 60 (1). pp. 181-183. ISSN 0907-4449
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Abstract
The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non-denaturing reversed-phase HPLC and was crystallized at room temperature by the hanging-drop vapour-diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 A under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P22(1)2(1) or P2(1)2(1)2, with unit-cell parameters a = 53.80, b = 119.39, c = 215.10 A.
| Item Type: | Article |
|---|---|
| Additional Information: | The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non-denaturing reversed-phase HPLC and was crystallized at room temperature by the hanging-drop vapour-diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 A under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P22(1)2(1) or P2(1)2(1)2, with unit-cell parameters a = 53.80, b = 119.39, c = 215.10 A. |
| Keywords: | levansucrase, structural biology |
| Subjects: | C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry |
| Divisions: | College of Sciences > Faculty of Science > School of Life Sciences |
| Depositing User: | Edward Taylor |
| Date Deposited: | 20 Sep 2012 16:40 |
| Last Modified: | 13 Mar 2013 09:13 |
| URI: | http://eprints.lincoln.ac.uk/id/eprint/6178 |
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