A 31P nuclear magnetic resonance study in vivo of metabolic abnormalities in rats with acute liver failure

Bates, T. E. and Williams, S. R. and Busza, A. L. and Gadian, D. G. and Proctor, E. (1988) A 31P nuclear magnetic resonance study in vivo of metabolic abnormalities in rats with acute liver failure. NMR in biomedicine, 1 (2). pp. 67-73. ISSN 0952-3480

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Full text URL: http://dx.doi.org/10.1002/nbm.1940010203

Abstract

31P NMR spectroscopy was used in conjunction with conventional biochemical techniques to study metabolic abnormalities in normal rats, in rats with CCl4-induced acute liver failure and in rats with paracetamol-induced acute liver failure. Studies were carried out before and after a metabolic challenge in the form of a fructose infusion. Prior to fructose infusion the ATP levels in the groups with acute liver failure were significantly lower than in control rats, despite their having a similar ATP/Pi ratio. Following a fructose infusion in control animals, the changes in phosphomonoesters, ATP and inorganic phosphate were consistent with previously reported findings. However, in both test groups the percentage changes in phosphomonoesters and ATP were significantly smaller. Intracellular pH declined in control animals, but did not change significantly in the animals with acute liver failure. Fructose clearance from the blood in control animals was not significantly different from that in animals with CCl4-induced acute liver failure. Metabolic changes measured using a horizontal magnet were very similar to those obtained with a vertical magnet.

Item Type:Article
Additional Information:31P NMR spectroscopy was used in conjunction with conventional biochemical techniques to study metabolic abnormalities in normal rats, in rats with CCl4-induced acute liver failure and in rats with paracetamol-induced acute liver failure. Studies were carried out before and after a metabolic challenge in the form of a fructose infusion. Prior to fructose infusion the ATP levels in the groups with acute liver failure were significantly lower than in control rats, despite their having a similar ATP/Pi ratio. Following a fructose infusion in control animals, the changes in phosphomonoesters, ATP and inorganic phosphate were consistent with previously reported findings. However, in both test groups the percentage changes in phosphomonoesters and ATP were significantly smaller. Intracellular pH declined in control animals, but did not change significantly in the animals with acute liver failure. Fructose clearance from the blood in control animals was not significantly different from that in animals with CCl4-induced acute liver failure. Metabolic changes measured using a horizontal magnet were very similar to those obtained with a vertical magnet.
Keywords:carbon tetrachloride, acute liver failure, Fructose, MRS, NMR Spectroscopy
Subjects:B Subjects allied to Medicine > B140 Neuroscience
B Subjects allied to Medicine > B200 Pharmacology, Toxicology and Pharmacy
Divisions:College of Science > School of Life Sciences
ID Code:5405
Deposited By: Timothy Bates
Deposited On:15 May 2012 21:09
Last Modified:15 May 2012 21:18

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