Assessment of energy metabolism in the developing brain following aglycemic hypoxia by 1H and 31P NMR

Brooks, K. J. and Clark, J. B. and Bates, T. E. (1996) Assessment of energy metabolism in the developing brain following aglycemic hypoxia by 1H and 31P NMR. Neurochemical Research, 21 (9). pp. 1089-1095. ISSN 0364-3190

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Abstract

The role played by external calcium and calcium channels in the recovery from aglycaemic hypoxia in cortical brain slices from 10-day old rats was investigated by 1H and 31P NMR. 30 minutes of aglycaemic hypoxia significantly decreased the levels of phosphocreatine (PCr), ATP, lactate and intracellular pH (pHi). After a 30 minute recovery period there was incomplete recovery of PCr and ATP with lactate increasing by 50% with pHi normal. When the aglycemic hypoxia was carried out in media which had no added calcium (approximately 10 microM) the PCr and ATP recovery was significantly greater. Application of diltiazem or verapamil but not nifedipine significantly improved the recovery from the aglycemic hypoxia. These data suggest that calcium influx through L-type voltage-gated calcium channels is involved in the ischemic damage in neonatal brain which manifests itself as a decrease in the energy state and an increase in lactate.

Item Type:Article
Additional Information:The role played by external calcium and calcium channels in the recovery from aglycaemic hypoxia in cortical brain slices from 10-day old rats was investigated by 1H and 31P NMR. 30 minutes of aglycaemic hypoxia significantly decreased the levels of phosphocreatine (PCr), ATP, lactate and intracellular pH (pHi). After a 30 minute recovery period there was incomplete recovery of PCr and ATP with lactate increasing by 50% with pHi normal. When the aglycemic hypoxia was carried out in media which had no added calcium (approximately 10 microM) the PCr and ATP recovery was significantly greater. Application of diltiazem or verapamil but not nifedipine significantly improved the recovery from the aglycemic hypoxia. These data suggest that calcium influx through L-type voltage-gated calcium channels is involved in the ischemic damage in neonatal brain which manifests itself as a decrease in the energy state and an increase in lactate.
Keywords:brain slices, aglycemia, hypoxia, 31P, 1H, NMR Spectroscopy
Subjects:A Medicine and Dentistry > A100 Pre-clinical Medicine
B Subjects allied to Medicine > B140 Neuroscience
Divisions:College of Science > School of Life Sciences
ID Code:5320
Deposited By: Timothy Bates
Deposited On:10 Jun 2012 21:24
Last Modified:10 Jun 2012 21:24

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