Nitric-oxide-induced inhibition of mitochondrial complexes following aglycaemic hypoxia in neonatal cortical rat brain slices

Brooks, K. J. and Hargreaves, I. P. and Bates, T. E. (2000) Nitric-oxide-induced inhibition of mitochondrial complexes following aglycaemic hypoxia in neonatal cortical rat brain slices. Developmental Neuroscience, 22 (5-6). pp. 359-65. ISSN 0378-5866

Full content URL: http://dx.doi.org/10.1159/000017461

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Item Type:Article
Item Status:Live Archive

Abstract

The effect of aglycaemic hypoxia (AH) on the activity of the mitochondrial respiratory chain complexes was measured in superfused neonatal cortical brain slices. After 30 min AH, there were no significant changes in the activities of complex I, II-III and IV or citrate synthase compared to controls. Following 30 min AH and a 30-min reperfusion period (with oxygen and glucose), the activities of complex II-III and complex IV were significantly reduced (by 25 and 17%, respectively). These reductions in enzyme activity were not abrogated by removing external calcium prior to and throughout AH, but could be reversed by the presence of the nitric oxide (NO) synthase inhibitor N omega-nitro-L-arginine during these periods. These data suggest that NO or an NO-derived species is involved in the decreases in mitochondrial enzyme activities observed after AH

Keywords:nitric oxide, Nitric Oxide Synthase, hypoxia, aglycemia, brain slices, neonatal
Subjects:B Subjects allied to Medicine > B131 Cellular Pathology
A Medicine and Dentistry > A100 Pre-clinical Medicine
B Subjects allied to Medicine > B140 Neuroscience
Divisions:College of Science > School of Life Sciences
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ID Code:5278
Deposited On:09 Jun 2013 17:41

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