Delayed hypothermia prevents decreases in N-acetylaspartate and reduced glutathione in the cerebral cortex of the neonatal pig following transient hypoxia-ischaemia

Brooks, Keith J and Hargreaves, Iain and Bhakoo, Kishore and Sellwood, Mark and O'Brien, Francis and Noone, Martina and Sakata, Yasuko and Cady, Ernest and Wylezinska, Marzena and Thornton, John and Ordidge, Roger and Nguyen, Quyen and Clemence, Matthew and Wyatt, John and Bates, Timothy E. (2002) Delayed hypothermia prevents decreases in N-acetylaspartate and reduced glutathione in the cerebral cortex of the neonatal pig following transient hypoxia-ischaemia. Neurochemical Research, 27 (12). pp. 1599-604. ISSN 0364-3190

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Item Type:Article
Item Status:Live Archive

Abstract

The effects of normothermia and delayed hypothermia on the levels of N-acetylaspartate (NAA), reduced glutathione (GSH) and the activities of mitochondrial complex I, II-III, IV and citrate synthase were measured in brain homogenates obtained from anaesthetized neonatal pigs following transient in vivo hypoxia-ischaemia. In the normothermic animals there was a significant decrease in complex I activity and in the levels of GSH and NAA when compared to the controls. Delayed hypothermia preserved NAA and GSH at control levels and enhanced the rate of complex II-III activity. There was correlation (R = 0.79) between GSH and NAA levels when data from all three experimental groups were analyzed. Citrate synthase activity was not significantly different in the three groups, indicating maintenance of mitochondrial integrity. These data suggest that delayed hypothermia affords protection of integrated mitochondrial function in the neonatal brain following transient hypoxia-ischaemia.

Keywords:neonatal, brain, Ischemia, cerebral ischemia, NAA, GSH, N-Acetylaspartate, glutatione, reperfusion, hypothermia
Subjects:B Subjects allied to Medicine > B131 Cellular Pathology
A Medicine and Dentistry > A100 Pre-clinical Medicine
B Subjects allied to Medicine > B140 Neuroscience
B Subjects allied to Medicine > B132 Pathobiology
A Medicine and Dentistry > A300 Clinical Medicine
Divisions:College of Science > School of Life Sciences
ID Code:5250
Deposited On:03 Jun 2013 21:54

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