A chicken bioreactor for efficient production of functional cytokines

Herron, Lissa R. and Pridans, Clare and Turnbull, Matthew L. and Smith, Nikki and Lillico, Simon and Sherman, Adrian and Gilhooley, Hazel J. and Wear, Martin and Kurian, Dominic and Papadakos, Grigorios and Digard, Paul and Hume, David A. and Gill, Andrew and Sang, Helen M. (2018) A chicken bioreactor for efficient production of functional cytokines. BMC Biotechnology, 18 (82). p. 82. ISSN 1472-6750

Full content URL: http://doi.org/10.1186/s12896-018-0495-1

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Abstract

The global market for protein drugs has the highest compound annual growth rate of any pharmaceutical class but their availability, especially outside of the US market, is compromised by the high cost of manufacture and validation compared to traditional chemical drugs. Improvements in transgenic technologies allow valuable proteins to be produced by genetically-modified animals; several therapeutic proteins from such animal bioreactors are already on the market after successful clinical trials and regulatory approval. Chickens have lagged behind mammals in bioreactor development, despite a number of potential advantages, due to the historic difficulty in producing transgenic birds, but the production of therapeutic proteins in egg white of transgenic chickens would substantially lower costs across the entire production cycle compared to traditional cell culture-based production systems. This could lead to more affordable treatments and wider markets, including in developing countries and for animal health applications. Here we report the efficient generation of new transgenic chicken lines to optimize protein production in eggs. As proof-of-concept, we describe the expression, purification and functional characterization of three pharmaceutical proteins, the human cytokine interferon α2a and two species-specific Fc fusions of the cytokine CSF1. Our work optimizes and validates a transgenic chicken system for the cost-effective production of pure, high quality, biologically active protein for therapeutics and other applications.

Keywords:Transgenic animals, Biologics, Recombinant protein, Cytokines, Animal Biotechnology
Subjects:C Biological Sciences > C560 Biotechnology
C Biological Sciences > C110 Applied Biology
F Physical Sciences > F165 Biomolecular Chemistry
C Biological Sciences > C760 Biomolecular Science
Divisions:College of Science
ID Code:34634
Deposited On:18 Feb 2019 12:51

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