Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D- arabinitol 1-phosphate in leaves of Phaseolus vulgaris L.

John Andralojc, P. and Keys, A.J. and Martindale, Wayne and Dawson, G.W. and Parry, M.A.J. (1996) Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D- arabinitol 1-phosphate in leaves of Phaseolus vulgaris L. Journal of Biological Chemistry, 271 (43). pp. 26803-26809. ISSN 00219258

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Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D- arabinitol 1-phosphate in leaves of Phaseolus vulgaris L.

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Abstract

1-14CHamamelose (2-hydroxymethyl-D-ribose) was synthesized by reaction of ribulose 5-phosphate with potassium 14Ccyanide, catalytic hydrogenation of the resulting cyanohydrin, and dephosphorylation of the product. Its identity was established by a chromatographic comparison with hamamelose isolated from the bark of witch hazel (Hamamelis virginiana L.). Following vacuum infiltration of the 1-14Chamamelose into leaf discs from Phaseolus vulgaris L., 14C-labeled 2-carboxy-D-arabinitol (CA) and 2- carboxy-D-arabinitol 1-phosphate (CA1P) were formed, in the dark. Conversion of hamamelose to both CA and CA1P in the leaf discs was inhibited by dithiothreitol and sodium fluoride, although at high concentrations of these inhibitors conversion into CA was still evident when conversion into CA1P was totally inhibited. Wheat (Triticum aestivum L.) leaves converted hamamelose into CA without formation of CA1P. Leaves from P. vulgaris contained 68 nmol · g-1 fresh weight of hamamelose in the light and 35 nmol · g-1 fresh weight in the dark. A pathway for the biosynthesis of CA1P from Calvin cycle intermediates is proposed which includes the sequence: hamamelose CA � CA1P.

Additional Information:cited By 12
Keywords:arabinitol, ribose, ribulosebisphosphate carboxylase, article, enzyme activity, enzyme phosphorylation, enzyme purification, enzyme synthesis, plant leaf, priority journal, Anions, Carbon Radioisotopes, Chromatography, High Pressure Liquid, Fabaceae, Hexoses, Pentosephosphates, Plant Leaves, Plants, Medicinal, Sugar Alcohols, Corylus americana, Glyptocephalus cynoglossus, Hamamelidaceae, Hamamelis virginiana, Phaseolus vulgaris, Triticum aestivum
Subjects:C Biological Sciences > C110 Applied Biology
C Biological Sciences > C230 Plant Biotechnology
Divisions:College of Science > National Centre for Food Manufacturing
ID Code:33777
Deposited On:28 Nov 2018 09:10

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