Crystallization of the NADP-dependent β-keto acylcarrier protein reductase from Brassica napus

Fisher, M. and Sedelnikova, S.E. and Martindale, Wayne and Thomas, N.C. and Simon, J.W. and Slabas, A.R. and Rafferty, J.B. (2000) Crystallization of the NADP-dependent β-keto acylcarrier protein reductase from Brassica napus. Acta Crystallographica Section D: Biological Crystallography, 56 (1). pp. 86-88. ISSN 09074449

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Crystallization of the NADP-dependent β-keto acylcarrier protein reductase from Brassica napus
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Abstract

The NADP-dependent β-keto acyl-carrier protein reductase (BKR) from Brassica napus has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol of average molecular weight 1500 as the precipitant. The crystals belong to the hexagonal space group P6422, with unit-cell parameters a = b = 129.9, c = 93.1 à , α = β = 90, γ = 120°. Calculated values for V(m), the use of rotation and translation functions and consideration of the packing suggest that the asymmetric unit contains a monomer. The crystals diffract to beyond 2.8 à resolution and are more amenable to X-ray diffraction analysis than those reported previously for the Escherichia coli enzyme. The structure determination of B. napus BKR will provide important insights into the catalytic mechanism of the enzyme and into the evolution of the fatty-acid elongation cycle by comparisons with the other oxidoreductase of the pathway, enoyl acyl-carrier protein reductase (ENR).

Additional Information:cited By 3
Keywords:3 oxoacyl (acyl carrier protein) reductase, 3-oxoacyl-(acyl-carrier-protein) reductase, alcohol dehydrogenase, recombinant protein, article, Brassica, chemistry, crystallization, enzymology, Escherichia coli, genetics, isolation and purification, X ray crystallography, Alcohol Oxidoreductases, Crystallography, X-Ray, Recombinant Proteins, Brassica napus
Subjects:C Biological Sciences > C710 Applied Molecular Biology, Biophysics and Biochemistry
Divisions:College of Science > National Centre for Food Manufacturing
ID Code:33773
Deposited On:27 Nov 2018 16:06

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