Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation

Burch, Hilary J and Contera, Sonia Antoranz and de Planque, Maurits R R and Grobert, Nicole and Ryan, J F (2008) Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation. Nanotechnology, 19 (38). p. 384001. ISSN 0957-4484

Full content URL: http://doi.org/10.1088/0957-4484/19/38/384001

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Item Type:Article
Item Status:Live Archive

Abstract

Relevant parameters for non-covalent protein functionalization of carbon nanotubes are
explored. Multiwalled carbon nanotubes are carboxylated and functionalized with
metalloproteins. Using atomic force microscopy (AFM) we quantitatively determine that
coverage with nitrogen-doped multiwalled carbon nanotubes is superior compared to coverage
with un-doped multiwalled carbon nanotubes, due to enhanced carboxylation. Conformational
analysis using a combination of AFM, antibody binding assays, circular dichroism and
UV–visible spectroscopy demonstrates that the metalloproteins retain their native structure
when adsorbed to nitrogen-doped multiwalled carbon nanotubes irrespective of their size,
charge or folding motif.

Additional Information:The final published version of this article can be accessed online at http://iopscience.iop.org/article/10.1088/0957-4484/19/38/384001/meta
Keywords:Multiwalled carbon nanotubes (MWCN), Doping
Subjects:F Physical Sciences > F310 Applied Physics
Divisions:College of Science > School of Chemistry
ID Code:33720
Deposited On:17 Oct 2018 08:26

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