Use of flow cytometry and total viable count to determine the effects of orange juice composition on the physiology of Escherichia coli

Anvarian, Amir and Smith, MP and Overton, TW (2018) Use of flow cytometry and total viable count to determine the effects of orange juice composition on the physiology of Escherichia coli. Food Science and Nutrition . ISSN 2048-7177

Full content URL: https://onlinelibrary.wiley.com/doi/full/10.1002/f...

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Use of flow cytometry and total viable count to determine the effects of orange juice composition on the physiology of Escherichia coli
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Abstract

Orange juice (OJ) contains numerous compounds some of which are known to play key roles in growth and survival of bacteria. This study aimed to investigate the effects of natural or processing‐induced variations in OJ composition on the physiology of Escherichia coli. OJ and model OJ (MOJ) samples containing various sugars, organic acids, amino acids, or ascorbic acid were inoculated with E. coli K‐12 MG1655 in different growth phases. The culturability, viability, and physiology of the cells were investigated during storage using plate counting and flow cytometry. Generally, stationary‐phase cells displayed the greatest survival in both MOJ and OJ. Increase in incubation temperature from 4 to 22.5ºC caused a significant decrease in both healthy and culturable cell populations. Supplementation of MOJ with ascorbic acid and amino acids increased both the viability and culturability of the cells. Similar trends were observed in amino acid‐supplemented OJ, albeit at a slower rate. In contrast, variations in sugar or organic acid composition had negligible effects on the physiological status of the cells. In summary, natural variation in ascorbic acid or amino acid concentrations could potentially have an adverse effect on the microbiological safety of orange juice.

Additional Information:© 2018 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords:amino acids, E. coli, flow cytometry, orange juice, viable but nonculturable
Subjects:D Veterinary Sciences, Agriculture and related subjects > D711 Agricultural Microbiology
C Biological Sciences > C590 Microbiology not elsewhere classified
C Biological Sciences > C510 Applied Microbiology
C Biological Sciences > C500 Microbiology
Divisions:College of Science > National Centre for Food Manufacturing
ID Code:33052
Deposited On:30 Aug 2018 07:49

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