Modular assembly of proteins on nanoparticles

Ma, Wenwei and Saccardo, Angela and Roccatano, Danilo and Aboagye-Mensah, Dorothy and Alkaseem, Mohammad and Jewkes, Matthew and Di Nezza, Francesca and Baron, Mark and Soloviev, Mikhail and Ferrari, Enrico (2018) Modular assembly of proteins on nanoparticles. Nature Communications, 9 (1). ISSN 2041-1723

Full content URL: http://doi.org/10.1038/s41467-018-03931-4

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Modular assembly of proteins on nanoparticles
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Abstract

Generally, the high diversity of protein properties necessitates the development of unique nanoparticle bio-conjugation methods, optimized for each different protein. Here we describe a universal bio-conjugation approach which makes use of a new recombinant fusion protein combining two distinct domains. The N-terminal part is Glutathione S-Transferase (GST) from Schistosoma japonicum, for which we identify and characterize the remarkable ability to bind gold nanoparticles (GNPs) by forming gold–sulfur bonds (Au–S). The C-terminal part of this multi-domain construct is the SpyCatcher from Streptococcus pyogenes, which provides the ability to capture recombinant proteins encoding a SpyTag. Here we show that SpyCatcher can be immobilized covalently on GNPs through GST without the loss of its full functionality. We then show that GST-SpyCatcher activated particles are able to covalently bind a SpyTag modified protein by simple mixing, through the spontaneous formation of an unusual isopeptide bond.

Keywords:Nanoparticles, Biomaterials proteins, Nanoscale materials
Subjects:C Biological Sciences > C720 Biological Chemistry
Divisions:College of Science > School of Life Sciences
ID Code:31706
Deposited On:16 Apr 2018 10:20

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