Re-engineering of the PAM1 phage display monoclonal antibody to produce a soluble, versatile anti-homogalacturonan scFv

Manfield, Iain W. and Bernal, Adriana J. and Møller, Isabel and McCartney, Lesley and Riess, Nicholas and Knox, J. Paul and Willats, William G.T. (2005) Re-engineering of the PAM1 phage display monoclonal antibody to produce a soluble, versatile anti-homogalacturonan scFv. Plant Science, 169 (6). pp. 1090-1095. ISSN 0168-9452

Full content URL: http://doi.org/10.1016/j.plantsci.2005.07.008

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Item Type:Article
Item Status:Live Archive

Abstract

Antibody phage display is an increasingly important alternative method for the production of monoclonal antibodies (mAbs) and involves the expression of antibody fragments (scFvs) at the surface of bacteriophage particles. We have previously used this technique to generate a phage mAb (PAM1phage) with specificity for the un-esterified regions of the homogalacturonan backbone of pectic polymers. Although phage particles are essential during mAb selection and amplification, their large size results in phage mAbs being poor probes for immunocytochemistry. In order to overcome this and to extend the utility of the PAM1 mAb, we describe here the production of a phage-free, soluble scFv version of the PAM1 mAb (PAM1scFv). Using the new PAM1scFv probe, the occurrence of the HG epitope recognized can now be localized with high resolution within micro-domains of plant cell walls.

Additional Information:The final published version is available online at https://www.sciencedirect.com/science/article/pii/S016894520500258X?via%3Dihub
Keywords:Phage display, Homogalacturonan, Monoclonal antibody, PAM1
Subjects:C Biological Sciences > C240 Plant Cell Science
Divisions:College of Science > School of Chemistry
ID Code:31320
Deposited On:23 Jul 2018 14:29

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