Production of N -acyl homoserine lactones by the sponge-associated marine actinobacteria Salinispora arenicola and Salinispora pacifica

Bose, Utpal and Ortori, Catharine A. and Sarmad, Sarir and Barrett, David A. and Hewavitharana, Amitha K. and Hodson, Mark P. and Fuerst, John A. and Shaw, P. Nicholas (2017) Production of N -acyl homoserine lactones by the sponge-associated marine actinobacteria Salinispora arenicola and Salinispora pacifica. FEMS Microbiology Letters, 364 (2). fnx002. ISSN 0378-1097

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Production of N-acyl homoserine lactones by the sponge-associated marine actinobacteria Salinispora arenicola and Salinispora pacifica

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Abstract

The structures of acyl homoserine lactone (AHL) compounds and their quantification were accomplished using an integrated liquid chromatography-mass spectrometry approach. The precursor and product ions, along with retention times of peaks, were searched against an in-house database of AHLs and structures confirmed by accurate mass and by comparison with authentic AHL standards. The two compounds, N-(3-oxodecanoyl)-L-homoserine lactone and N-(3-oxododecanoyl)-L-homoserine lactone, were characterised and quantified in Salinispora sp. cultures.

Keywords:Keywords: marine bacteria, homoserine lactone, liquid chromatography, mass spectrometry
Subjects:C Biological Sciences > C160 Marine/Freshwater Biology
Divisions:College of Science > School of Chemistry
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ID Code:31310
Deposited On:14 Mar 2018 16:39

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