Intestinal fatty acid-binding protein and gut permeability responses to exercise

March, Daniel and Marchbank, Tania and Playford, Raymond and Jones, Arwel and Thatcher, Rhys and Davison, Glen (2017) Intestinal fatty acid-binding protein and gut permeability responses to exercise. European Journal of Applied Physiology, 117 (5). pp. 931-941. ISSN 1439-6319

Full content URL: https://doi.org/10.1007/s00421-017-3582-4

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Abstract

Purpose

Intestinal cell damage due to physiological stressors (e.g. heat, oxidative, hypoperfusion/ischaemic) may contribute to increased intestinal permeability. The aim of this study was to assess changes in plasma intestinal fatty acid-binding protein (I-FABP) in response to exercise (with bovine colostrum supplementation, Col, positive control) and compare this to intestinal barrier integrity/permeability (5 h urinary lactulose/rhamnose ratio, L/R).

Methods

In a double-blind, placebo-controlled, crossover design, 18 males completed two experimental arms (14 days of 20 g/day supplementation with Col or placebo, Plac). For each arm participants performed two baseline (resting) intestinal permeability assessments (L/R) pre-supplementation and one post-exercise following supplementation. Blood samples were collected pre- and post-exercise to determine I-FABP concentration.

Results

Two-way repeated measures ANOVA revealed an arm × time interaction for L/R and I-FABP (P < 0.001). Post hoc analyses showed urinary L/R increased post-exercise in Plac (273% of pre, P < 0.001) and Col (148% of pre, P < 0.001) with post-exercise values significantly lower with Col (P < 0.001). Plasma I-FABP increased post-exercise in Plac (191% of pre-exercise, P = 0.002) but not in the Col arm (107%, P = 0.862) with post-exercise values significantly lower with Col (P = 0.013). Correlations between the increase in I-FABP and L/R were evident for visit one (P = 0.044) but not visit two (P = 0.200) although overall plots/patterns do appear similar for each.

Conclusion

These findings suggest that exercise-induced intestinal cellular damage/injury is partly implicated in changes in permeability but other factors must also contribute.

Keywords:Strenuous exercise, Intestinal permeability, Core temperature, Bovine colostrum, Cell damage, Cellular injury, Urinary L/R
Subjects:B Subjects allied to Medicine > B120 Physiology
B Subjects allied to Medicine > B400 Nutrition
Divisions:College of Social Science > Lincoln Institute of Health
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ID Code:30541
Deposited On:01 Mar 2018 13:17

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