Molecular epidemiology and phylogeny reveal complex spatial dynamics in areas where canine parvovirus is endemic

Clegg, S.R. and Coyne, K.P. and Parker, J. and Dawson, S. and Godsall, S.A. and Pinchbeck, G. and Cripps, P.J. and Gaskell, R.M. and Radford, A.D. (2011) Molecular epidemiology and phylogeny reveal complex spatial dynamics in areas where canine parvovirus is endemic. Journal of Virology, 85 (15). pp. 7892-7899. ISSN 0022538X

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Item Type:Article
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Abstract

Canine parvovirus type 2 (CPV-2) is a severe enteric pathogen of dogs, causing high mortality in unvaccinated dogs. After emerging, CPV-2 spread rapidly worldwide. However, there is now some evidence to suggest that international transmission appears to be more restricted. In order to investigate the transmission and evolution of CPV-2 both nationally and in relation to the global situation, we have used a long-range PCR to amplify and sequence the full VP2 gene of 150 canine parvoviruses obtained from a large cross-sectional sample of dogs presenting with severe diarrhea to veterinarians in the United Kingdom, over a 2-year period. Among these 150 strains, 50 different DNA sequence types (S) were identified, and apart from one case, all appeared unique to the United Kingdom. Phylogenetic analysis provided clear evidence for spatial clustering at the international level and for the first time also at the national level, with the geographical range of some sequence types appearing to be highly restricted within the United Kingdom. Evolution of the VP2 gene in this data set was associated with a lack of positive selection. In addition, the majority of predicted amino acid sequences were identical to those found elsewhere in the world, suggesting that CPV VP2 has evolved a highly fit conformation. Based on typing systems using key amino acid mutations, 43 of viruses were CPV-2a, and 57 CPV-2b, with no type 2 or 2c found. However, phylogenetic analysis suggested complex antigenic evolution of this virus, with both type 2a and 2b viruses appearing polyphyletic. As such, typing based on specific amino acid mutations may not reflect the true epidemiology of this virus. The geographical restriction that we observed both within the United Kingdom and between the United Kingdom and other countries, together with the lack of CPV-2c in this population, strongly suggests the spread of CPV within its population may be heterogeneously subject to limiting factors. This cross-sectional study of national and global CPV phylogeographic segregation reveals a substantially more complex epidemic structure than previously described. © 2011, American Society for Microbiology.

Additional Information:cited By 22 The final published version of this article is available online at http://jvi.asm.org/content/85/15/7892
Keywords:article, binding affinity, canine parvovirus type 2, controlled study, cross-sectional study, gene sequence, geographic distribution, homozygosity, molecular dynamics, molecular epidemiology, molecular evolution, nonhuman, nucleotide sequence, Parvovirus, phylogeny, polymerase chain reaction, priority journal, sequence alignment, virus strain, virus transmission, Animals, Base Sequence, DNA Primers, Dog Diseases, Dogs, Great Britain, Parvoviridae Infections, Parvovirus, Canine, Prevalence, Canine parvovirus, Canine parvovirus 2, Canis familiaris
Subjects:C Biological Sciences > C522 Veterinary Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:29868
Deposited On:01 Aug 2018 09:48

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