High-level association of bovine digital dermatitis treponema spp. with contagious ovine digital dermatitis lesions and presence of fusobacterium necrophorum and dichelobacter nodosus

Sullivan, L. E. and Clegg, S. R. and Angell, J. W. and Newbrook, K. and Blowey, R. W. and Carter, S. D. and Bell, J. and Duncan, J. S. and Grove-White, D. H. and Murray, R. D. and Evans, N. J. (2015) High-level association of bovine digital dermatitis treponema spp. with contagious ovine digital dermatitis lesions and presence of fusobacterium necrophorum and dichelobacter nodosus. Journal of Clinical Microbiology, 53 (5). pp. 1628-1638. ISSN 0095-1137

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Abstract

Contagious ovine digital dermatitis (CODD) is an important foot disease in sheep, with significant animal welfare and economic implications. It is thought that CODD emerged from bovine digital dermatitis (BDD) via treponemal bacteria. With wildlife species such as elk now suffering a CODD-like disease, it is imperative to clarify these disease etiologies. A large investigation into treponemal association with CODD is warranted. CODD lesions (n=58) and healthy sheep foot tissues (n=56) were analyzed by PCR for the three BDD-associated Treponema phylogroups and two other lameness-associated bacteria, Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was also attempted on CODD lesions. "Treponema medium/Treponema vincentii-like," "Treponema phagedenis-like," and Treponema pedis spirochetes were identified in 39/58 (67), 49/58 (85), and 41/58 (71) of CODD lesions, respectively. One or more BDD-associated Treponema phylogroups were detected in 100 of CODD lesions. Healthy foot tissues did not amplify BDD-associated Treponema phylogroup DNA. D. nodosus and F. necrophorum were present in 34/58 (59) and 41/58 (71) of CODD lesions and 22/56 (39) and 5/56 (9) of healthy foot tissues, respectively. Thirty-two spirochetes were isolated from CODD lesions, with representatives clustering with, and indistinguishable from, each of the three BDD-associated Treponema phylogroups based on 16S rRNA gene comparisons. This study for the first time demonstrates a high-level association for BDD treponeme phylogroups in CODD and their absence from healthy tissues, supporting the hypothesis that BDD treponemes play a primary causative role in CODD and confirming that the specific PCR assays are an effective differential diagnostic tool for CODD. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Keywords:RNA 16S, bacterial DNA, ribosome DNA, animal experiment, animal tissue, Article, bacterium culture, bacterium detection, bacterium identification, contagious ovine digital dermatitis, controlled study, Dichelobacter nodosus, digital dermatitis, Fusobacterium necrophorum, nonhuman, polymerase chain reaction, priority journal, sheep, spirochete, Treponema, animal, bovine, chemistry, cluster analysis, DNA sequence, domestic sheep, genetics, isolation and purification, microbiology, molecular genetics, phylogeny, Treponema, Animalia, Bovinae, Ovis, Ovis aries, Spirochaetales, Treponema phagedenis, Animals, Cattle, DNA, Bacterial, DNA, Ribosomal, Molecular Sequence Data, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Sheep, Domestic
Subjects:D Veterinary Sciences, Agriculture and related subjects > D420 Livestock
C Biological Sciences > C522 Veterinary Microbiology
D Veterinary Sciences, Agriculture and related subjects > D328 Animal Welfare
Divisions:College of Science > School of Life Sciences
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ID Code:29866
Deposited On:20 Feb 2018 12:22

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