In vitro susceptibility of contagious ovine digital dermatitis associated Treponema spp. isolates to antimicrobial agents in the UK

Angell, J. W. and Clegg, S. R. and Sullivan, L. E. and Duncan, J. S. and Grove-White, D. H. and Carter, S. D. and Evans, N. J. (2015) In vitro susceptibility of contagious ovine digital dermatitis associated Treponema spp. isolates to antimicrobial agents in the UK. Veterinary Dermatology, 26 (6). 484-487 and e114. ISSN 0959-4493

Full content URL: https://doi.org/10.1111/vde.12269

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Abstract

Background: Contagious ovine digital dermatitis (CODD) is an important cause of infectious lameness in sheep in the UK and Ireland and has a severe impact on the welfare of affected individuals. The three treponemal phylogroups Treponema medium/Treponema vincentii-like, Treponema phagedenis-like and Treponema pedis spirochaetes have been associated with clinical CODD lesions and are considered to be a necessary cause of disease. There are scant data on the antimicrobial susceptibility of the treponemes cultured from CODD lesions. Objective: The aim of this study was to determine in vitro the miniumum inhibitory concentration/ minimum bactericidal concentration (MIC/MBC) of antimicrobials used in the sheep industry for isolates of the three CODD associated treponeme phylogroups T. medium/T. vincentii-like, T. phagedenis-like and T. pedis. Animals: Twenty treponeme isolates; from 19 sheep with clinical CODD lesions. Methods: A microdilution method was used to determine in vitro the MIC/MBC of 10 antimicrobial agents for 20 treponeme isolates (five T. medium/T. vincentii-like, 10 T. phagedenis-like and five T. pedis). The antimicrobials tested were penicillin G, amoxicillin, oxytetracycline, tilmicosin, lincomycin, spectinomycin, tylosin, tildipirosin, tulathromycin and gamithromycin. Results: The treponeme isolates tested showed low MICs and MBCs to all 10 antimicrobials tested. They were most susceptible to gamithromycin and tildipirosin (MIC90: 0.0469 mg/L), and were least susceptible to lincomycin, spectinomycin and oxytetracycline (MIC90: 48 mg/L, 24 mg/L and 3 mg/L, respectively). Conclusions: These data are comparable to in vitro antimicrobial susceptibility data for treponemes cultured from bovine digital dermatitis lesions. Dependent on local licensing, penicillin and tilmicosin appear to be the best candidates for future in vivo studies. © 2015 ESVD & ACVD.

Additional Information:cited By 2
Keywords:antiinfective agent, animal, digital dermatitis, drug effects, Great Britain, microbial sensitivity test, microbiology, sheep, Sheep Diseases, Treponema, Treponemal Infections, veterinary, Animals, Anti-Bacterial Agents, Microbial Sensitivity Tests
Subjects:D Veterinary Sciences, Agriculture and related subjects > D420 Livestock
C Biological Sciences > C522 Veterinary Microbiology
Divisions:College of Science > School of Life Sciences
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ID Code:29863
Deposited On:20 Feb 2018 13:00

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