A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions

Sullivan, L. E. and Evans, N. J. and Blowey, R. W. and Grove-White, D. H. and Clegg, S. R. and Duncan, J. S. and Carter, S. D. (2015) A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions. Veterinary Microbiology, 178 (1-2). pp. 77-87. ISSN 0378-1135

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Abstract

Bovine digital dermatitis (BDD) is an infective foot disease commonly reported in dairy cattle where Treponema are considered as the primary causative infectious agents. There still remains little definitive information on the etiology of BDD in beef cattle suggesting further investigations are warranted. Beef BDD lesions (. n=. 34) and healthy beef foot tissues (. n=. 38) were analysed by PCR for three BDD-associated Treponema phylogroups and also for Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was attempted on all BDD lesion samples. One or more BDD-associated Treponema phylogroups were detected in 100 of beef BDD lesions. ". Treponema medium/Treponema vincentii-like", ". Treponema phagedenis-like" and Treponema pedis spirochetes were identified in 27/34 (79), 31/34 (91) and 24/34 (71) of BDD lesions, respectively. No BDD-associated treponeme DNA was amplified from beef healthy foot tissues. D. nodosus and F. necrophorum were present in 24/34 (71) and 15/34 (44) of lesions and 10/38 (26) and 12/38 (32) of healthy foot tissues, respectively. Twenty spirochetes were isolated from beef BDD lesions; 19 were representatives of the three BDD-associated Treponema phylogroups. One spirochete isolate shared less than 97 16S rRNA gene similarity to the three cultivable BDD-associated Treponema phylogroups and therefore may represent a novel taxa of Treponema. Upon comparison, sheep contagious ovine digital dermatitis (CODD), dairy cattle and beef cattle BDD lesions appear to have extremely similar bacteriological data and therefore provides evidence of a shared etiopathogenesis posing concerns for cross-species transmission. © 2015 Elsevier B.V.

Keywords:bacterial RNA, ribosome RNA, RNA 16S, animal tissue, Article, bacterial transmission, bacterium isolation, beef cattle, comparative study, controlled study, dairy cattle, Dichelobacter nodosus, digital dermatitis, Fusobacterium necrophorum, molecular epidemiology, nonhuman, pathogenesis, phylogeny, RNA sequence, sheep, Treponema, animal, bovine, Cattle Diseases, genetics, microbiology, polymerase chain reaction, procedures, Sheep Diseases, spirochete, Treponemal Infections, veterinary, Bos, Bovinae, Ovis, Ovis aries, Spirochaetales, Treponema phagedenis, Animals, Cattle, RNA, Ribosomal, 16S
Subjects:D Veterinary Sciences, Agriculture and related subjects > D420 Livestock
D Veterinary Sciences, Agriculture and related subjects > D711 Agricultural Microbiology
D Veterinary Sciences, Agriculture and related subjects > D320 Animal Health
Divisions:College of Science > School of Life Sciences
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ID Code:29862
Deposited On:05 Dec 2017 20:09

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