Detection and isolation of digital dermatitis treponemes from bovine pressure sores

Clegg, S. R. and Crosby-Durrani, H. E. and Bell, J. and Blundell, R. and Blowey, R. W. and Carter, S. D. and Evans, N. J. (2016) Detection and isolation of digital dermatitis treponemes from bovine pressure sores. Journal of Comparative Pathology, 154 (4). pp. 273-282. ISSN 0021-9975

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Abstract

Pressure sores cause severe pain and discomfort in hospitalized people and in farmed cattle and are often infected with unknown bacteria. Pressure sores occur on the upper legs of 6-10 of recumbent cattle and are generally considered to be caused by constant pressure, commonly on bony areas of the limbs. This study analyzed pressure sores taken from the upper limbs of 14 cattle using isolation in culture and nested polymerase chain reaction (PCR) to detect treponemes associated with digital dermatitis (DD). A 100 association of DD treponemes with the pressure sores was demonstrated, but treponemes were shown not to be part of the normal skin microbiota. Immunohistochemistry showed an association of DD treponemes with lesions and particularly with the hair follicles in lesions, identifying the bacteria deep within wounds, thereby suggesting that they could contribute to lesion pathogenesis. The bacteria isolated from the pressure sore lesions were similar or identical on analysis of the 16S rRNA gene to those found in DD foot lesions in cattle, suggesting the same bacteria can infect multiple lesions. Indeed, the results of this study suggest that these spirochaetal bacteria may be expanding in host range and in their ability to colonize different tissues and contribute to a range of disease manifestations in farm animals. © 2016 Elsevier Ltd.

Additional Information:cited By 2
Keywords:bacterial RNA, ribosome RNA, animal tissue, Article, bacterial colonization, bacterial gene, bacterium culture, bacterium detection, bacterium isolation, bovine, decubitus, digital dermatitis, disease association, gene sequence, hair follicle, histopathology, host range, immunohistochemistry, nonhuman, pathogenesis, phylogeny, polymerase chain reaction, priority journal, skin flora, Treponema, upper limb, animal, cattle disease, complication, female, microbiology, treponematosis, veterinary, Animals, Cattle, Cattle Diseases, Pressure Ulcer, Treponemal Infections
Subjects:D Veterinary Sciences, Agriculture and related subjects > D320 Animal Health
D Veterinary Sciences, Agriculture and related subjects > D711 Agricultural Microbiology
Divisions:College of Science > School of Life Sciences
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ID Code:29856
Deposited On:06 Dec 2017 10:30

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