Treponema ruminis sp. nov., a spirochaete isolated from the bovine rumen

Newbrook, Kerry and Staton, Gareth J. and Clegg, Simon R. and Birtles, Richard J. and Carter, Stuart D. and Evans, Nicholas J. (2017) Treponema ruminis sp. nov., a spirochaete isolated from the bovine rumen. International Journal of Systematic and Evolutionary Microbiology, 67 (5). pp. 1349-1354. ISSN 1466-5026

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Abstract

A novel bacterium, strain Ru1T, was encountered during a survey of spirochaetes living in the gastrointestinal tract of ruminants. Comparative analysis of 16S rRNA gene sequence data indicated that strain Ru1T clustered within the genus Treponema but shared at most 86.1 sequence similarity with other recognised species of the genus Treponema. Further phylogenetic analysis based on partial recombinase A (recA) gene sequence comparisons, together with phenotypic characterization, also demonstrated the divergence of strain Ru1T from other recognised species of the genus Treponema. Microscopically, strain Ru1T appeared as a very small, highly motile, helical spirochaete with eight periplasmic flagella in a 4: 8: 4 arrangement. It exhibited C8 esterase lipase, leucine arylamidase, β-galactosidase and β-glucosidase activity. A distinctive, serum-independent growth pattern was also observed, characterized by colonies with an absence of the local haemolysis that is typical of many pathogenic treponemes. On the basis of these data, strain Ru1T is considered to represent a novel species of the genus Treponema, for which the name Treponema ruminis sp. nov. is proposed. The type strain is Ru1T (=DSM 103462T=NCTC 13847T). © 2017 IUMS.

Keywords:aryl acylamidase, beta galactosidase, beta glucosidase, esterase, RecA protein, triacylglycerol lipase, bacterial DNA, RNA 16S, Article, bacterial flagellum, bacterium isolate, bovine, enzyme activity, gene sequence, nonhuman, nucleotide sequence, phylogeny, priority journal, rumen, spirochete, Treponema, Treponema ruminis, animal, bacterium identification, classification, DNA base composition, DNA sequence, genetics, isolation and purification, male, microbiology, United Kingdom, Animals, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial, RNA, Ribosomal, 16S, Sequence Analysis, DNA, United Kingdom
Subjects:D Veterinary Sciences, Agriculture and related subjects > D711 Agricultural Microbiology
C Biological Sciences > C522 Veterinary Microbiology
C Biological Sciences > C530 Bacteriology
Divisions:College of Science > School of Life Sciences
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ID Code:29853
Deposited On:01 Mar 2018 11:49

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