Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate

Tregaskes, Clive A. and Glansbeek, Harrie L. and Gill, Andrew C. and Hunt, Lawrence G. and Burnside, Joan and Young, John R. (2004) Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate. Developmental and Comparative Immunology, 29 (4). pp. 361-374. ISSN 0145-305X

Full content URL: http://doi.org/10.1016/j.dci.2004.09.001

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Abstract

Signals delivered by the CD40 ligand, CD154, have crucial roles in immune responses in mammals, being required for development of germinal centres, maturation of T-dependent antibody responses, and generation of B-cell memory. To determine whether these functions were conserved in a non-mammalian species, a putative chicken CD154 cDNA was used to make an oligomeric fusion protein, and to raise monoclonal antibodies. The antibodies detected surface expression on activated T-cells. The fusion protein detected expression of a receptor on B-cells, thrombocytes and macrophages. Biological effects of the fusion protein included induction of NO synthesis in a macrophage cell line, enhancement of splenic B-cell survival, and induction of apoptosis in a bursal lymphoma cell line. These observations demonstrated substantial functional equivalence with mammalian CD154 and thus provided evidence for the early evolutionary emergence of the set of functions associated with this molecule, and its central role in the vertebrate immune system.

Keywords:CD154, CD40 ligand, CD40 antigen, Fusion protein, TNFR, Evolution, Chicken
Subjects:D Veterinary Sciences, Agriculture and related subjects > D900 Others in Veterinary Sciences, Agriculture and related subjects
C Biological Sciences > C770 Biophysical Science
C Biological Sciences > C760 Biomolecular Science
Divisions:College of Science
ID Code:29563
Deposited On:01 Dec 2017 08:56

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