Prion protein-specific antibodies that detect multiple TSE agents with high sensitivity

McCutcheon, Sandra and Langeveld, Jan P. M. and Tan, Boon Chin and Gill, Andrew C. and de Wolf, Christopher and Martin, Stuart and Gonzalez, Lorenzo and Alibhai, James and Blanco, A. Richard Alejo and Campbell, Lauren and Hunter, Nora and Houston, E. Fiona (2014) Prion protein-specific antibodies that detect multiple TSE agents with high sensitivity. PLoS ONE, 9 (3). e91143. ISSN 1932-6203

Full content URL: http://doi.org/10.1371/journal.pone.0091143

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Abstract

This paper describes the generation, characterisation and potential applications of a panel of novel anti-prion protein monoclonal antibodies (mAbs). The mAbs were generated by immunising PRNP null mice, using a variety of regimes, with a truncated form of recombinant ovine prion protein spanning residues 94–233. Epitopes of specific antibodies were mapped using solid-phase Pepscan analysis and clustered to four distinct regions within the PrP molecule. We have demonstrated the utility of these antibodies by use of Western blotting and immunohistochemistry in tissues from a range of different species affected by transmissible spongiform encephalopathy (TSE). In comparative tests against extensively-used and widely-published, commercially available antibodies, similar or improved results can be obtained using these new mAbs, specifically in terms of sensitivity of detection. Since many of these antibodies recognise native PrPC, they could also be applied to a broad range of immunoassays such as flow cytometry, DELFIA analysis or immunoprecipitation. We are using these reagents to increase our understanding of TSE pathogenesis and for use in potential diagnostic screening assays.

Keywords:prion, transmissible spongiform encephalopathies, scrapie, antibody
Subjects:B Subjects allied to Medicine > B140 Neuroscience
C Biological Sciences > C550 Immunology
D Veterinary Sciences, Agriculture and related subjects > D320 Animal Health
Divisions:College of Science
ID Code:29467
Deposited On:20 Nov 2017 13:32

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